8a3b: Difference between revisions
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==Cryo-EM structure of mouse Pannexin 1 purified in Salipro nanoparticles== | |||
<StructureSection load='8a3b' size='340' side='right'caption='[[8a3b]], [[Resolution|resolution]] 3.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8a3b]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A3B FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a3b OCA], [https://pdbe.org/8a3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a3b RCSB], [https://www.ebi.ac.uk/pdbsum/8a3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a3b ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PANX1_MOUSE PANX1_MOUSE] Ion channel involved in a variety of physiological functions such as blood pressure regulation, apoptotic cell clearance and oogenesis (PubMed:30814251). Forms anion-selective channels with relatively low conductance and an order of permeabilities: nitrate>iodide>chlroride>>aspartate=glutamate=gluconate (PubMed:22311122). Can release ATP upon activation through phosphorylation or cleavage at C-terminus (PubMed:32238926). May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis (By similarity).[UniProtKB:Q96RD7]<ref>PMID:22311122</ref> <ref>PMID:30814251</ref> <ref>PMID:32238926</ref> During apoptosis and after cleavage by caspases of the C-terminal tail, acts as a plasma membrane channel which mediates the regulated release of find-me signals, such as nucleotides ATP and UTP, and selective plasme membrane permeability.<ref>PMID:32238926</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here, with the pannexin1 channel as a case study, we demonstrate the applicability of this method for structure-function analysis using SPR and cryo-EM. | |||
Direct cell extraction of membrane proteins for structure-function analysis.,Drulyte I, Gutgsell AR, Lloris-Garcera P, Liss M, Geschwindner S, Radjainia M, Frauenfeld J, Loving R Sci Rep. 2023 Jan 25;13(1):1420. doi: 10.1038/s41598-023-28455-w. PMID:36697499<ref>PMID:36697499</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Drulyte | <div class="pdbe-citations 8a3b" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Pannexin|Pannexin]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mus musculus]] | |||
[[Category: Drulyte I]] | |||
Latest revision as of 06:43, 24 July 2024
Cryo-EM structure of mouse Pannexin 1 purified in Salipro nanoparticles
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