8d8k: Difference between revisions
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==Yeast mitochondrial small subunit assembly intermediate (State 2)== | |||
<StructureSection load='8d8k' size='340' side='right'caption='[[8d8k]], [[Resolution|resolution]] 3.13Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8d8k]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D8K FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.13Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d8k OCA], [https://pdbe.org/8d8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d8k RCSB], [https://www.ebi.ac.uk/pdbsum/8d8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d8k ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RT22_YEAST RT22_YEAST] Mitochondrial ribosome (mitoribosome) assembly factor (PubMed:36482135, PubMed:38199006). Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body (PubMed:36482135). Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity (By similarity).[UniProtKB:Q9H7H0]<ref>PMID:36482135</ref> <ref>PMID:38199006</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is essential for ATP production and cellular metabolism(1). Here we used cryo-electron microscopy to determine nine structures of native yeast and human mitoribosomal small subunit assembly intermediates, illuminating the mechanistic basis for how GTPases are used to control early steps of decoding centre formation, how initial rRNA folding and processing events are mediated, and how mitoribosomal proteins have active roles during assembly. Furthermore, this series of intermediates from two species with divergent mitoribosomal architecture uncovers both conserved principles and species-specific adaptations that govern the maturation of mitoribosomal small subunits in eukaryotes. By revealing the dynamic interplay between assembly factors, mitoribosomal proteins and rRNA that are required to generate functional subunits, our structural analysis provides a vignette for how molecular complexity and diversity can evolve in large ribonucleoprotein assemblies. | |||
Principles of mitoribosomal small subunit assembly in eukaryotes.,Harper NJ, Burnside C, Klinge S Nature. 2023 Feb;614(7946):175-181. doi: 10.1038/s41586-022-05621-0. Epub 2022 , Dec 8. PMID:36482135<ref>PMID:36482135</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8d8k" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharomyces cerevisiae]] | |||
[[Category: Burnside C]] | |||
[[Category: Harper NJ]] | |||
[[Category: Klinge S]] | |||
Latest revision as of 15:02, 13 August 2026
Yeast mitochondrial small subunit assembly intermediate (State 2)
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