8dku: Difference between revisions
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==CryoEM structure of the A. aeolicus WzmWzt transporter bound to the native O antigen== | |||
<StructureSection load='8dku' size='340' side='right'caption='[[8dku]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8dku]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DKU FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UBH:3-O-methyl-alpha-D-rhamnopyranose'>UBH</scene>, <scene name='pdbligand=UBO:beta-D-rhamnopyranose'>UBO</scene>, <scene name='pdbligand=XXR:6-DEOXY-ALPHA-D-MANNOPYRANOSE'>XXR</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dku OCA], [https://pdbe.org/8dku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dku RCSB], [https://www.ebi.ac.uk/pdbsum/8dku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dku ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/O67181_AQUAE O67181_AQUAE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
O antigens are ubiquitous protective extensions of lipopolysaccharides in the extracellular leaflet of the Gram-negative outer membrane. Following biosynthesis in the cytosol, the lipid-linked polysaccharide is transported to the periplasm by the WzmWzt ABC transporter. Often, O antigen secretion requires the chemical modification of its elongating terminus, which the transporter recognizes via a carbohydrate-binding domain (CBD). Here, using components from A. aeolicus, we identify the O antigen structure with methylated mannose or rhamnose as its cap. Crystal and cryo electron microscopy structures reveal how WzmWzt recognizes this cap between its carbohydrate and nucleotide-binding domains in a nucleotide-free state. ATP binding induces drastic conformational changes of its CBD, terminating interactions with the O antigen. ATPase assays and site directed mutagenesis reveal reduced hydrolytic activity upon O antigen binding, likely to facilitate polymer loading into the ABC transporter. Our results elucidate critical steps in the recognition and translocation of polysaccharides by ABC transporters. | |||
Molecular basis for polysaccharide recognition and modulated ATP hydrolysis by the O antigen ABC transporter.,Spellmon N, Muszynski A, Gorniak I, Vlach J, Hahn D, Azadi P, Zimmer J Nat Commun. 2022 Sep 5;13(1):5226. doi: 10.1038/s41467-022-32597-2. PMID:36064941<ref>PMID:36064941</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Muszynski | <div class="pdbe-citations 8dku" style="background-color:#fffaf0;"></div> | ||
[[Category: Spellmon | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Aquifex aeolicus VF5]] | |||
[[Category: Large Structures]] | |||
[[Category: Muszynski A]] | |||
[[Category: Spellmon N]] | |||
[[Category: Vlach J]] | |||
[[Category: Zimmer J]] | |||
Latest revision as of 05:22, 12 June 2024
CryoEM structure of the A. aeolicus WzmWzt transporter bound to the native O antigen
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