8dq8: Difference between revisions
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New page: '''Unreleased structure''' The entry 8dq8 is ON HOLD until Paper Publication Authors: Wu, K., Dulchavsky, M., Li, J., Bardwell, J.C.A. Description: The structure of NicA2 variant F104L... |
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The | ==The structure of NicA2 variant F104L/A107T/S146I/G317D/H368R/L449V/N462S in complex with N-methylmyosmine== | ||
<StructureSection load='8dq8' size='340' side='right'caption='[[8dq8]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8dq8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida_S16 Pseudomonas putida S16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DQ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DQ8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | |||
[[Category: | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=NCT:(S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE'>NCT</scene></td></tr> | ||
[[Category: | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dq8 OCA], [https://pdbe.org/8dq8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dq8 RCSB], [https://www.ebi.ac.uk/pdbsum/8dq8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dq8 ProSAT]</span></td></tr> | ||
[[Category: Dulchavsky | </table> | ||
[[Category: | == Function == | ||
[[Category: | [https://www.uniprot.org/uniprot/NICA2_PSEP6 NICA2_PSEP6] Involved in nicotine degradation (PubMed:24204321, PubMed:26237398, PubMed:29812904, PubMed:33432238). Catalyzes the conversion of nicotine to N-methylmyosmine (PubMed:24204321, PubMed:29812904, PubMed:33432238, PubMed:33464876). N-methylmyosmine undergoes spontaneous hydrolysis to form pseudooxynicotine (PN) (PubMed:24204321). S-nicotine is the optimal substrate (PubMed:29812904). Has lower activity with some nicotine analogs, but shows no activity towards neurotransmitters, including serotonin, dopamine, and norepinephrine, nicotine metabolites and common neuroactive drugs (PubMed:29812904). The enzyme is stereospecific with poor activity with (R)-nicotine as the substrate (PubMed:29812904). The c-type cytochrome protein CycN is the physiological electron acceptor (PubMed:33432238). O(2) is a poor electron acceptor (PubMed:33432238, PubMed:33464876).<ref>PMID:24204321</ref> <ref>PMID:26237398</ref> <ref>PMID:29812904</ref> <ref>PMID:33432238</ref> <ref>PMID:33464876</ref> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas putida S16]] | |||
[[Category: Bardwell JCA]] | |||
[[Category: Dulchavsky M]] | |||
[[Category: Li J]] | |||
[[Category: Wu K]] | |||
Latest revision as of 09:33, 4 March 2026
The structure of NicA2 variant F104L/A107T/S146I/G317D/H368R/L449V/N462S in complex with N-methylmyosmine
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