8dqk: Difference between revisions
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New page: '''Unreleased structure''' The entry 8dqk is ON HOLD Authors: Ho, R., Purushotham, P., Zimmer, J. Description: Intermediate resolution structure of barley (1,3:1,4)-beta-glucan synthas... |
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==Intermediate resolution structure of barley (1,3;1,4)-beta-glucan synthase CslF6.== | |||
<StructureSection load='8dqk' size='340' side='right'caption='[[8dqk]], [[Resolution|resolution]] 4.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8dqk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DQK FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dqk OCA], [https://pdbe.org/8dqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dqk RCSB], [https://www.ebi.ac.uk/pdbsum/8dqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dqk ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/F2DMH9_HORVV F2DMH9_HORVV] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Mixed-linkage (1,3;1,4)-beta-glucans, which are widely distributed in cell walls of the grasses, are linear glucose polymers containing predominantly (1,4)-beta-linked glucosyl units interspersed with single (1,3)-beta-linked glucosyl units. Their distribution in cereal grains and unique structures are important determinants of dietary fibers that are beneficial to human health. We demonstrate that the barley cellulose synthase-like CslF6 enzyme is sufficient to synthesize a high-molecular weight (1,3;1,4)-beta-glucan in vitro. Biochemical and cryo-electron microscopy analyses suggest that CslF6 functions as a monomer. A conserved "switch motif" at the entrance of the enzyme's transmembrane channel is critical to generate (1,3)-linkages. There, a single-point mutation markedly reduces (1,3)-linkage formation, resulting in the synthesis of cellulosic polysaccharides. Our results suggest that CslF6 monitors the orientation of the nascent polysaccharide's second or third glucosyl unit. Register-dependent interactions with these glucosyl residues reposition the polymer's terminal glucosyl unit to form either a (1,3)- or (1,4)-beta-linkage. | |||
Mechanism of mixed-linkage glucan biosynthesis by barley cellulose synthase-like CslF6 (1,3;1,4)-beta-glucan synthase.,Purushotham P, Ho R, Yu L, Fincher GB, Bulone V, Zimmer J Sci Adv. 2022 Nov 11;8(45):eadd1596. doi: 10.1126/sciadv.add1596. Epub 2022 Nov , 11. PMID:36367939<ref>PMID:36367939</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8dqk" style="background-color:#fffaf0;"></div> | ||
[[Category: Purushotham | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Hordeum vulgare]] | |||
[[Category: Large Structures]] | |||
[[Category: Ho R]] | |||
[[Category: Purushotham P]] | |||
[[Category: Zimmer J]] | |||
Latest revision as of 05:22, 12 June 2024
Intermediate resolution structure of barley (1,3;1,4)-beta-glucan synthase CslF6.
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