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New page: left|200px<br /> <applet load="1ft8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ft8, resolution 3.15Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:1ft8.gif|left|200px]]<br />
<applet load="1ft8" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ft8, resolution 3.15&Aring;" />
'''CRYSTAL STRUCTURE OF THE RNA-BINDING DOMAIN OF THE MRNA EXPORT FACTOR TAP'''<br />


==Overview==
==CRYSTAL STRUCTURE OF THE RNA-BINDING DOMAIN OF THE MRNA EXPORT FACTOR TAP==
Human TAP is implicated in mRNA nuclear export and is used by simian type, D retroviruses to export their unspliced genomic RNA to the cytoplasm of, the host cell. We have determined the crystal structure of the minimal TAP, fragment that binds the constitutive transport element (CTE) of retroviral, RNAs. Unexpectedly, we find the fragment consists of a ribonucleoprotein, (RNP) domain, which is not identifiable by its sequence, and a, leucine-rich repeat (LRR) domain. The non-canonical RNP domain functions, as the general RNA-binding portion of the fragment. The LRR domain is, required in cis to the RNP domain for CTE RNA binding. The structural and, biochemical properties of the domains point to a remarkable similarity, with the U2B"(RNP)-U2A'(LRR) spliceosomal heterodimer. Our in vitro and in, vivo functional studies using structure-based mutants suggest that a, phylogenetically conserved surface of the LRR domain of TAP may have, different roles in the export of viral and cellular RNAs.
<StructureSection load='1ft8' size='340' side='right'caption='[[1ft8]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
 
== Structural highlights ==
==Disease==
<table><tr><td colspan='2'>[[1ft8]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FT8 FirstGlance]. <br>
Known diseases associated with this structure: Bare lymphocyte syndrome, type I OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=170260 170260]]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ft8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ft8 OCA], [https://pdbe.org/1ft8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ft8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ft8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ft8 ProSAT]</span></td></tr>
==About this Structure==
</table>
1FT8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FT8 OCA].  
== Function ==
 
[https://www.uniprot.org/uniprot/NXF1_HUMAN NXF1_HUMAN] Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5.<ref>PMID:9660949</ref> <ref>PMID:19165146</ref>
==Reference==
== Evolutionary Conservation ==
The structure of the mRNA export factor TAP reveals a cis arrangement of a non-canonical RNP domain and an LRR domain., Liker E, Fernandez E, Izaurralde E, Conti E, EMBO J. 2000 Nov 1;19(21):5587-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11060011 11060011]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/1ft8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ft8 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Conti, E.]]
[[Category: Conti E]]
[[Category: Fernandez, E.]]
[[Category: Fernandez E]]
[[Category: Izaurralde, E.]]
[[Category: Izaurralde E]]
[[Category: Liker, E.]]
[[Category: Liker E]]
[[Category: rbd) and leucine-rich-repeat (lrr) domains]]
[[Category: ribonucleoprotein (rnp]]
[[Category: rrm]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:57:16 2007''

Latest revision as of 07:19, 7 February 2024

CRYSTAL STRUCTURE OF THE RNA-BINDING DOMAIN OF THE MRNA EXPORT FACTOR TAP

1ft8, resolution 3.15Å

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