3vis: Difference between revisions

Latest revision as of 09:48, 30 October 2024

Crystal structure of cutinase Est119 from Thermobifida alba AHK119

Structural highlights

3vis is a 2 chain structure with sequence from Thermobifida alba. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.76Å
Ligands:	PE4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PETH2_THEAE Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle (PubMed:20393707, PubMed:22183084, PubMed:25910960, PubMed:33387709). Shows esterase activity towards p-nitrophenol-linked aliphatic esters (pNP-aliphatic esters) (PubMed:20393707, PubMed:22183084, PubMed:25910960, PubMed:33387709). Capable of degrading the plastic poly(ethylene terephthalate) (PET), the most abundant polyester plastic in the world (By similarity). Can also depolymerize the synthetic polyesters poly(epsilon-caprolactone) (PCL), poly(butylene succinate-co-adipate) (PBSA), poly(butylene succinate) (PBS), and poly(lactic acid) (PLA) (PubMed:20393707, PubMed:22183084).[UniProtKB:D4Q9N1]^[1] ^[2] ^[3] ^[4]

References

↑ Hu X, Thumarat U, Zhang X, Tang M, Kawai F. Diversity of polyester-degrading bacteria in compost and molecular analysis of a thermoactive esterase from Thermobifida alba AHK119. Appl Microbiol Biotechnol. 2010 Jun;87(2):771-9. PMID:20393707 doi:10.1007/s00253-010-2555-x
↑ Thumarat U, Nakamura R, Kawabata T, Suzuki H, Kawai F. Biochemical and genetic analysis of a cutinase-type polyesterase from a thermophilic Thermobifida alba AHK119. Appl Microbiol Biotechnol. 2012 Jul;95(2):419-30. PMID:22183084 doi:10.1007/s00253-011-3781-6
↑ Thumarat U, Kawabata T, Nakajima M, Nakajima H, Sugiyama A, Yazaki K, Tada T, Waku T, Tanaka N, Kawai F. Comparison of genetic structures and biochemical properties of tandem cutinase-type polyesterases from Thermobifida alba AHK119. J Biosci Bioeng. 2015 Nov;120(5):491-7. PMID:25910960 doi:10.1016/j.jbiosc.2015.03.006
↑ Zhang Z, Wang W, Li D, Xiao J, Wu L, Geng X, Wu G, Zeng Z, Hu J. Decolorization of molasses alcohol wastewater by thermophilic hydrolase with practical application value. Bioresour Technol. 2021 Mar;323:124609. PMID:33387709 doi:10.1016/j.biortech.2020.124609

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OCA

[1] Hu X, Thumarat U, Zhang X, Tang M, Kawai F. Diversity of polyester-degrading bacteria in compost and molecular analysis of a thermoactive esterase from Thermobifida alba AHK119. Appl Microbiol Biotechnol. 2010 Jun;87(2):771-9. PMID:20393707 doi:10.1007/s00253-010-2555-x

[2] Thumarat U, Nakamura R, Kawabata T, Suzuki H, Kawai F. Biochemical and genetic analysis of a cutinase-type polyesterase from a thermophilic Thermobifida alba AHK119. Appl Microbiol Biotechnol. 2012 Jul;95(2):419-30. PMID:22183084 doi:10.1007/s00253-011-3781-6

[3] Thumarat U, Kawabata T, Nakajima M, Nakajima H, Sugiyama A, Yazaki K, Tada T, Waku T, Tanaka N, Kawai F. Comparison of genetic structures and biochemical properties of tandem cutinase-type polyesterases from Thermobifida alba AHK119. J Biosci Bioeng. 2015 Nov;120(5):491-7. PMID:25910960 doi:10.1016/j.jbiosc.2015.03.006

[4] Zhang Z, Wang W, Li D, Xiao J, Wu L, Geng X, Wu G, Zeng Z, Hu J. Decolorization of molasses alcohol wastewater by thermophilic hydrolase with practical application value. Bioresour Technol. 2021 Mar;323:124609. PMID:33387709 doi:10.1016/j.biortech.2020.124609

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[2]

[3]

[4]

@@ Line 3: / Line 3: @@
 <StructureSection load='3vis' size='340' side='right'caption='[[3vis]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3vis]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinobifida_alba"_locci_et_al._1967 "actinobifida alba" locci et al. 1967]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VIS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3vis]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_alba Thermobifida alba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VIS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PE4:2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>PE4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">est2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53522 "Actinobifida alba" Locci et al. 1967])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PE4:2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>PE4</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vis OCA], [https://pdbe.org/3vis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vis RCSB], [https://www.ebi.ac.uk/pdbsum/3vis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vis ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PETH2_THEAE PETH2_THEAE] Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle (PubMed:20393707, PubMed:22183084, PubMed:25910960, PubMed:33387709). Shows esterase activity towards p-nitrophenol-linked aliphatic esters (pNP-aliphatic esters) (PubMed:20393707, PubMed:22183084, PubMed:25910960, PubMed:33387709). Capable of degrading the plastic poly(ethylene terephthalate) (PET), the most abundant polyester plastic in the world (By similarity). Can also depolymerize the synthetic polyesters poly(epsilon-caprolactone) (PCL), poly(butylene succinate-co-adipate) (PBSA), poly(butylene succinate) (PBS), and poly(lactic acid) (PLA) (PubMed:20393707, PubMed:22183084).[UniProtKB:D4Q9N1]<ref>PMID:20393707</ref> <ref>PMID:22183084</ref> <ref>PMID:25910960</ref> <ref>PMID:33387709</ref>
+== References ==
+<references/>
 __TOC__
 </StructureSection>
-[[Category: Actinobifida alba locci et al. 1967]]
 [[Category: Large Structures]]
-[[Category: Karatani, H]]
+[[Category: Thermobifida alba]]
-[[Category: Kawai, F]]
+[[Category: Karatani H]]
-[[Category: Kitadokoro, K]]
+[[Category: Kawai F]]
-[[Category: Nakamura, R]]
+[[Category: Kitadokoro K]]
-[[Category: Nishimura, K]]
+[[Category: Nakamura R]]
-[[Category: Suzuki, H]]
+[[Category: Nishimura K]]
-[[Category: Thumarat, U]]
+[[Category: Suzuki H]]
-[[Category: Alpha/beta-hydrolase fold]]
+[[Category: Thumarat U]]
-[[Category: Esterase]]
-[[Category: Hydrolase]]
-[[Category: Polyethylene terephthalate]]

3vis: Difference between revisions

Latest revision as of 09:48, 30 October 2024

Crystal structure of cutinase Est119 from Thermobifida alba AHK119

Structural highlights

Function

References

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