3wjk: Difference between revisions

Latest revision as of 13:12, 8 November 2023

Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli

Structural highlights

3wjk is a 2 chain structure with sequence from Escherichia coli O104:H4 str. 2009EL-2071. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Octaprenyl pyrophosphate synthase (OPPs) catalyzes consecutive condensation reactions of one allylic substrate farnesyl pyrophosphate (FPP) and five homoallylic substrate isopentenyl pyrophosphate (IPP) molecules to form a C40 long-chain product OPP, which serves as a side chain of ubiquinone and menaquinone. OPPs belongs to the trans-prenyltransferase class of proteins. The structures of OPPs from Escherichia coli were solved in the apo-form as well as in complexes with IPP and a FPP thio-analog, FsPP, at resolutions of 2.2 to 2.6 A, and revealed the detailed interactions between the ligands and enzyme. At the bottom of the active-site tunnel, M123 and M135 act in concert to form a wall which determines the final chain length. These results represent the first ligand-bound crystal structures of a long-chain trans-prenyltransferase and provide new information on the mechanisms of catalysis and product chain elongation. (c) Proteins 2014;. (c) 2014 Wiley Periodicals, Inc.

Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from escherichia coli reveal the catalytic and chain-length determining mechanisms.,Han X, Chen CC, Kuo CJ, Huang CH, Zheng Y, Ko TP, Zhu Z, Feng X, Wang K, Oldfield E, Wang AH, Liang PH, Guo RT, Ma Y Proteins. 2014 Jun 4. doi: 10.1002/prot.24618. PMID:24895191^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Han X, Chen CC, Kuo CJ, Huang CH, Zheng Y, Ko TP, Zhu Z, Feng X, Wang K, Oldfield E, Wang AH, Liang PH, Guo RT, Ma Y. Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from escherichia coli reveal the catalytic and chain-length determining mechanisms. Proteins. 2014 Jun 4. doi: 10.1002/prot.24618. PMID:24895191 doi:https://dx.doi.org/10.1002/prot.24618

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OCA

[1] Han X, Chen CC, Kuo CJ, Huang CH, Zheng Y, Ko TP, Zhu Z, Feng X, Wang K, Oldfield E, Wang AH, Liang PH, Guo RT, Ma Y. Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from escherichia coli reveal the catalytic and chain-length determining mechanisms. Proteins. 2014 Jun 4. doi: 10.1002/prot.24618. PMID:24895191 doi:https://dx.doi.org/10.1002/prot.24618

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='3wjk' size='340' side='right'caption='[[3wjk]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3wjk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Eco1e Eco1e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WJK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3wjk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O104:H4_str._2009EL-2071 Escherichia coli O104:H4 str. 2009EL-2071]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WJK FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wjn|3wjn]], [[3wjo|3wjo]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">O3O_22640 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1133853 ECO1E])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjk OCA], [https://pdbe.org/3wjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wjk RCSB], [https://www.ebi.ac.uk/pdbsum/3wjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wjk ProSAT]</span></td></tr>
 </table>
@@ Line 21: / Line 20: @@
 __TOC__
 </StructureSection>
-[[Category: Eco1e]]
+[[Category: Escherichia coli O104:H4 str. 2009EL-2071]]
 [[Category: Large Structures]]
-[[Category: Chen, C C]]
+[[Category: Chen CC]]
-[[Category: Feng, X]]
+[[Category: Feng X]]
-[[Category: Guo, R T]]
+[[Category: Guo RT]]
-[[Category: Han, X]]
+[[Category: Han X]]
-[[Category: Huang, C H]]
+[[Category: Huang CH]]
-[[Category: Ko, T P]]
+[[Category: Ko TP]]
-[[Category: Kuo, C J]]
+[[Category: Kuo CJ]]
-[[Category: Liang, P H]]
+[[Category: Liang PH]]
-[[Category: Ma, Y H]]
+[[Category: Ma YH]]
-[[Category: Oldfield, E]]
+[[Category: Oldfield E]]
-[[Category: Zheng, Y]]
+[[Category: Zheng Y]]
-[[Category: Zhu, Z]]
+[[Category: Zhu Z]]
-[[Category: Prenyltransferase]]
-[[Category: Product chain length]]
-[[Category: Site-directed mutagenesis]]
-[[Category: Transferase]]

3wjk: Difference between revisions

Latest revision as of 13:12, 8 November 2023

Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli

Structural highlights

Publication Abstract from PubMed

References

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