1go7: Difference between revisions

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New page: left|200px<br /> <applet load="1go7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1go7, resolution 2.10Å" /> '''THE METZINCIN'S MET...
 
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[[Image:1go7.gif|left|200px]]<br />
<applet load="1go7" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1go7, resolution 2.10&Aring;" />
'''THE METZINCIN'S METHIONINE: PRTC M226C-E189K DOUBLE MUTANT'''<br />


==Overview==
==The metzincin's methionine: PrtC M226C-E189K double mutant==
Serralysins are a family of metalloproteases secreted by Gram-negative, bacteria into the medium in the form of inactive zymogens. Usually, all, serralysin secretors have on the same operon a gene coding for a, periplasmic 10-kDa protein, which is an inhibitor of the secreted, protease. The recent characterization of the inhibitor of the alkaline, protease from Pseudomonas aeruginosa revealed a surprisingly low, dissociation constant of 4 pm, contrary to earlier studies on homologous, systems, where inhibition constants in the microm range were reported. To, approach a more accurate understanding, the crystal structure of the, complex between inhibitor and protease from P. aeruginosa was determined, at 1.74 A resolution and refined to R(free) = 0.204. The structure, reported here shows ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11445573 (full description)]]
<StructureSection load='1go7' size='340' side='right'caption='[[1go7]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1go7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GO7 FirstGlance]. <br>
1GO7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]] with CA, ZN and PO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GO7 OCA]].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1go7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1go7 OCA], [https://pdbe.org/1go7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1go7 RCSB], [https://www.ebi.ac.uk/pdbsum/1go7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1go7 ProSAT]</span></td></tr>
Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond., Hege T, Feltzer RE, Gray RD, Baumann U, J Biol Chem. 2001 Sep 14;276(37):35087-92. Epub 2001 Jul 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11445573 11445573]
</table>
[[Category: Erwinia chrysanthemi]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/PRTC_DICCH PRTC_DICCH]  
[[Category: Hege, T.]]
== Evolutionary Conservation ==
[[Category: CA]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: PO4]]
Check<jmol>
[[Category: ZN]]
  <jmolCheckbox>
[[Category: hydrolase]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/1go7_consurf.spt"</scriptWhenChecked>
[[Category: metalloprotease]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: protease]]
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 18:08:56 2007''
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1go7 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Dickeya chrysanthemi]]
[[Category: Large Structures]]
[[Category: Hege T]]

Latest revision as of 11:23, 27 March 2024

The metzincin's methionine: PrtC M226C-E189K double mutant

1go7, resolution 2.10Å

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