8dfv: Difference between revisions

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'''Unreleased structure'''


The entry 8dfv is ON HOLD  until 2024-06-22
==Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex IIa==
<StructureSection load='8dfv' size='340' side='right'caption='[[8dfv]], [[Resolution|resolution]] 3.06&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8dfv]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DFV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.06&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dfv OCA], [https://pdbe.org/8dfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dfv RCSB], [https://www.ebi.ac.uk/pdbsum/8dfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dfv ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding domain (dsRBD) proteins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 that show how Dicer-1 and its partner Loqs‑PB cooperate (1) before binding pre-miRNA, (2) after binding and in a catalytically competent state, (3) after nicking one arm of the pre-miRNA, and (4) following complete dicing and initial product release. Our reconstructions suggest that pre-miRNA binds a rare, open conformation of the Dicer‑1⋅Loqs‑PB heterodimer. The Dicer-1 dsRBD and three Loqs‑PB dsRBDs form a tight belt around the pre-miRNA, distorting the RNA helix to place the scissile phosphodiester bonds in the RNase III active sites. Pre-miRNA cleavage shifts the dsRBDs and partially closes Dicer-1, which may promote product release. Our data suggest a model for how the Dicer‑1⋅Loqs‑PB complex affects a complete cycle of pre-miRNA recognition, stepwise endonuclease cleavage, and product release.


Authors: Jouravleva, K., Golovenko, D., Demo, G., Dutcher, R.C., Tanaka Hall, T.M., Zamore, P.D., Korostelev, A.A.
Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB.,Jouravleva K, Golovenko D, Demo G, Dutcher RC, Hall TMT, Zamore PD, Korostelev AA Mol Cell. 2022 Nov 3;82(21):4049-4063.e6. doi: 10.1016/j.molcel.2022.09.002. Epub , 2022 Sep 30. PMID:36182693<ref>PMID:36182693</ref>


Description: Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB -complex IIa
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Tanaka Hall, T.M]]
<div class="pdbe-citations 8dfv" style="background-color:#fffaf0;"></div>
[[Category: Demo, G]]
== References ==
[[Category: Golovenko, D]]
<references/>
[[Category: Korostelev, A.A]]
__TOC__
[[Category: Dutcher, R.C]]
</StructureSection>
[[Category: Jouravleva, K]]
[[Category: Drosophila melanogaster]]
[[Category: Zamore, P.D]]
[[Category: Large Structures]]
[[Category: Demo G]]
[[Category: Dutcher RC]]
[[Category: Golovenko D]]
[[Category: Jouravleva K]]
[[Category: Korostelev AA]]
[[Category: Tanaka Hall TM]]
[[Category: Zamore PD]]
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