1i0m: Difference between revisions

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[[Image:1i0m.gif|left|200px]]


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==1.05 A STRUCTURE OF THE A-DECAMER GCGTATACGC WITH A SINGLE 2'-O-FLUOROETHYL THYMINE IN PLACE OF T6, HIGH RB-SALT==
The line below this paragraph, containing "STRUCTURE_1i0m", creates the "Structure Box" on the page.
<StructureSection load='1i0m' size='340' side='right'caption='[[1i0m]], [[Resolution|resolution]] 1.05&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1i0m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I0M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I0M FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=125:2-O-FLUOROETHYL-5-METHYL-URIDINE-5-MONOPHOSPHATE'>125</scene>, <scene name='pdbligand=RB:RUBIDIUM+ION'>RB</scene></td></tr>
{{STRUCTURE_1i0m|  PDB=1i0m  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i0m OCA], [https://pdbe.org/1i0m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i0m RCSB], [https://www.ebi.ac.uk/pdbsum/1i0m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i0m ProSAT]</span></td></tr>
 
</table>
'''1.05 A STRUCTURE OF THE A-DECAMER GCGTATACGC WITH A SINGLE 2'-O-FLUOROETHYL THYMINE IN PLACE OF T6, HIGH RB-SALT'''
__TOC__
 
</StructureSection>
 
[[Category: Large Structures]]
==Overview==
[[Category: Egli M]]
The observation of light metal ions in nucleic acids crystals is generally a fortuitous event. Sodium ions in particular are notoriously difficult to detect because their X-ray scattering contributions are virtually identical to those of water and Na(+.)O distances are only slightly shorter than strong hydrogen bonds between well-ordered water molecules. We demonstrate here that replacement of Na(+) by K(+), Rb(+) or Cs(+) and precise measurements of anomalous differences in intensities provide a particularly sensitive method for detecting alkali metal ion-binding sites in nucleic acid crystals. Not only can alkali metal ions be readily located in such structures, but the presence of Rb(+) or Cs(+) also allows structure determination by the single wavelength anomalous diffraction technique. Besides allowing identification of high occupancy binding sites, the combination of high resolution and anomalous diffraction data established here can also pinpoint binding sites that feature only partial occupancy. Conversely, high resolution of the data alone does not necessarily allow differentiation between water and partially ordered metal ions, as demonstrated with the crystal structure of a DNA duplex determined to a resolution of 0.6 A.
[[Category: Howard A]]
 
[[Category: Maier MA]]
==About this Structure==
[[Category: Manoharan M]]
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I0M OCA].  
[[Category: Minasov G]]
 
[[Category: Prakash TP]]
==Reference==
[[Category: Tereshko V]]
Detection of alkali metal ions in DNA crystals using state-of-the-art X-ray diffraction experiments., Tereshko V, Wilds CJ, Minasov G, Prakash TP, Maier MA, Howard A, Wawrzak Z, Manoharan M, Egli M, Nucleic Acids Res. 2001 Mar 1;29(5):1208-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11222771 11222771]
[[Category: Wawrzak Z]]
[[Category: Egli, M.]]
[[Category: Wilds CJ]]
[[Category: Howard, A.]]
[[Category: Maier, M A.]]
[[Category: Manoharan, M.]]
[[Category: Minasov, G.]]
[[Category: Prakash, T P.]]
[[Category: Tereshko, V.]]
[[Category: Wawrzak, Z.]]
[[Category: Wilds, C J.]]
[[Category: A-form double helix]]
[[Category: Modified sugar]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:26:13 2008''
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