1i8t: Difference between revisions

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[[Image:1i8t.jpg|left|200px]]


<!--
==STRUCTURE OF UDP-GALACTOPYRANOSE MUTASE FROM E.COLI==
The line below this paragraph, containing "STRUCTURE_1i8t", creates the "Structure Box" on the page.
<StructureSection load='1i8t' size='340' side='right'caption='[[1i8t]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1i8t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I8T FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
{{STRUCTURE_1i8t| PDB=1i8t |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8t OCA], [https://pdbe.org/1i8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i8t RCSB], [https://www.ebi.ac.uk/pdbsum/1i8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GLF_ECOLI GLF_ECOLI] Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF) (By similarity).<ref>PMID:8576037</ref> <ref>PMID:11448178</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/1i8t_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i8t ConSurf].
<div style="clear:both"></div>


'''STRCUTURE OF UDP-GALACTOPYRANOSE MUTASE FROM E.COLI'''
==See Also==
 
*[[UDP-galactopyranose mutase 3D structures|UDP-galactopyranose mutase 3D structures]]
 
== References ==
==Overview==
<references/>
Uridine diphosphogalactofuranose (UDP-Galf ) is the precursor of the d-galactofuranose (Galf ) residues found in bacterial and parasitic cell walls, including those of many pathogens, such as Mycobacterium tuberculosis and Trypanosoma cruzi. UDP-Galf is made from UDP-galactopyranose (UDP-Galp) by the enzyme UDP-galactopyranose mutase (mutase). The mutase enzyme is essential for the viability of mycobacteria and is not found in humans, making it a viable therapeutic target. The mechanism by which mutase achieves the unprecedented ring contraction of a nonreducing sugar is unclear. We have solved the crystal structure of Escherichia coli mutase to 2.4 A resolution. The novel structure shows that the flavin nucleotide is located in a cleft lined with conserved residues. Site-directed mutagenesis studies indicate that this cleft contains the active site, with the sugar ring of the substrate UDP-galactose adjacent to the exposed isoalloxazine ring of FAD. Assay results establish that the enzyme is active only when flavin is reduced. We conclude that mutase most likely functions by transient reduction of substrate.
__TOC__
 
</StructureSection>
==About this Structure==
1I8T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8T OCA].
 
==Reference==
UDP-galactopyranose mutase has a novel structure and mechanism., Sanders DA, Staines AG, McMahon SA, McNeil MR, Whitfield C, Naismith JH, Nat Struct Biol. 2001 Oct;8(10):858-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11573090 11573090]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: UDP-galactopyranose mutase]]
[[Category: McMahon SA]]
[[Category: McMahon, S A.]]
[[Category: McNeil MR]]
[[Category: McNeil, M R.]]
[[Category: Naismith JH]]
[[Category: Naismith, J H.]]
[[Category: Sanders DAR]]
[[Category: Sanders, D A.R.]]
[[Category: Staines AG]]
[[Category: Staines, A G.]]
[[Category: Whitfield C]]
[[Category: Whitfield, C.]]
[[Category: Contractase]]
[[Category: Fad]]
[[Category: Mutase]]
[[Category: Rossman fold]]
[[Category: Udp-galactopyranose]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:42:59 2008''

Latest revision as of 07:32, 7 February 2024

STRUCTURE OF UDP-GALACTOPYRANOSE MUTASE FROM E.COLI

1i8t, resolution 2.40Å

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