8edk: Difference between revisions
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New page: '''Unreleased structure''' The entry 8edk is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Structure of C. elegans UNC-6 LamN and EGF domains== | |||
<StructureSection load='8edk' size='340' side='right'caption='[[8edk]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8edk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EDK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EDK FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8edk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8edk OCA], [https://pdbe.org/8edk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8edk RCSB], [https://www.ebi.ac.uk/pdbsum/8edk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8edk ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/UNC6_CAEEL UNC6_CAEEL] Component of an extracellular matrix cue that guides dorsoventral migrations on the epidermis (PubMed:8861903). Required for the guidance of pioneer axons and migrating cells along the body wall (PubMed:28846083, PubMed:8332188). In particular, it is required for the guidance of axons from neurons, including SubL neurons and AIY interneurons, into the nerve ring (PubMed:28846083, PubMed:9473333). During gonad morphogenesis, involved in distal tip cell (DTC) migration from the dorsal side of the hermaphrodite body to the midbody to allow for formation of gonad arms (PubMed:17716643, PubMed:22732572). Its association with either unc-40 or unc-5 receptors will lead to axon attraction or repulsion, respectively (PubMed:8332188, PubMed:8861903, PubMed:9473333). Involved in dendritic morphogenesis; may act by association with unc-40 at the tips of growing dendrites for interaction with unc-5 on the apposing branch to induce mutual repulsion (PubMed:22426253). Involved in the positioning of ray 1, the most anterior ray sensilium, in the male tail (PubMed:24004945). Required for the formation of synapses between the AVA interneurons and the PHB sensory neurons (PubMed:29742100).<ref>PMID:17716643</ref> <ref>PMID:22426253</ref> <ref>PMID:22732572</ref> <ref>PMID:24004945</ref> <ref>PMID:28846083</ref> <ref>PMID:29742100</ref> <ref>PMID:8332188</ref> <ref>PMID:8861903</ref> <ref>PMID:9473333</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Netrins dictate attractive and repulsive responses during axon growth and cell migration, where the presence of the receptor Uncoordinated-5 (UNC-5) on target cells results in repulsion. Here, we showed that UNC-5 is a heparin-binding protein, determined its structure bound to a heparin fragment, and could modulate UNC-5-heparin affinity using a directed evolution platform or structure-based rational design. We demonstrated that UNC-5 and UNC-6/netrin form a large, stable, and rigid complex in the presence of heparin, and heparin and UNC-5 exclude the attractive UNC-40/DCC receptor from binding to UNC-6/netrin to a large extent. Caenorhabditis elegans with a heparin-binding-deficient UNC-5 fail to establish proper gonad morphology due to abrogated cell migration, which relies on repulsive UNC-5 signaling in response to UNC-6. Combining UNC-5 mutations targeting heparin and UNC-6/netrin contacts results in complete cell migration and axon guidance defects. Our findings establish repulsive netrin responses to be mediated through a glycosaminoglycan-regulated macromolecular complex. | |||
Structural insights into the formation of repulsive netrin guidance complexes.,Priest JM, Nichols EL, Smock RG, Hopkins JB, Mendoza JL, Meijers R, Shen K, Ozkan E Sci Adv. 2024 Feb 16;10(7):eadj8083. doi: 10.1126/sciadv.adj8083. Epub 2024 Feb , 16. PMID:38363837<ref>PMID:38363837</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8edk" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Caenorhabditis elegans]] | |||
[[Category: Large Structures]] | |||
[[Category: Ozkan E]] | |||
[[Category: Priest JM]] | |||
