1sa3: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1sa3]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Moraxella_sp. Moraxella sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SA3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1sa3]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Moraxella_sp. Moraxella sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SA3 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sa3 OCA], [https://pdbe.org/1sa3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sa3 RCSB], [https://www.ebi.ac.uk/pdbsum/1sa3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sa3 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sa3 OCA], [https://pdbe.org/1sa3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sa3 RCSB], [https://www.ebi.ac.uk/pdbsum/1sa3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sa3 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/T2M1_MORSP T2M1_MORSP]] Recognizes the double-stranded sequence CCGG and cleaves after C-1.
[https://www.uniprot.org/uniprot/T2M1_MORSP T2M1_MORSP] Recognizes the double-stranded sequence CCGG and cleaves after C-1.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Most well-known restriction endonucleases recognize palindromic DNA sequences and are classified as Type IIP. Due to the recognition and cleavage symmetry, Type IIP enzymes are usually found to act as homodimers in forming 2-fold symmetric enzyme-DNA complexes. Here we report an asymmetric complex of the Type IIP restriction enzyme MspI in complex with its cognate recognition sequence. Unlike any other Type IIP enzyme reported to date, an MspI monomer and not a dimer binds to a palindromic DNA sequence. The enzyme makes specific contacts with all 4 base pairs in the recognition sequence, by six direct and five water-mediated hydrogen bonds and numerous van der Waal contacts. This MspI-DNA structure represents the first example of asymmetric recognition of a palindromic DNA sequence by two different structural motifs in one polypeptide. A few possible pathways are discussed for MspI to cut both strands of DNA, either as a monomer or dimer.
 
An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site.,Xu QS, Kucera RB, Roberts RJ, Guo HC Structure. 2004 Sep;12(9):1741-7. PMID:15341737<ref>PMID:15341737</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1sa3" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>

Latest revision as of 08:29, 14 February 2024

An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site

1sa3, resolution 1.95Å

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