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[[Image:1ihw.gif|left|200px]]
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{{STRUCTURE_1ihw|  PDB=1ihw  |  SCENE=  }}
'''SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, 40 STRUCTURES'''


==SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, 40 STRUCTURES==
<StructureSection load='1ihw' size='340' side='right'caption='[[1ihw]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ihw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IHW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ihw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ihw OCA], [https://pdbe.org/1ihw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ihw RCSB], [https://www.ebi.ac.uk/pdbsum/1ihw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ihw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/POL_HV1Z6 POL_HV1Z6] Integrase performs the integration of the newly synthesized dsDNA copy of the viral genome into the host chromosome. The integrated DNA is called provirus.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The solution structure of the DNA binding domain of HIV-1 integrase (residues 220-270) has been determined by multidimensional NMR spectroscopy. The protein is a dimer in solution, and each subunit is composed of a five-stranded beta-barrel with a topology very similar to that of the SH3 domain. The dimer is formed by a stacked beta-interface comprising strands 2, 3, and 4, with the two triple-stranded antiparallel beta-sheets, one from each subunit, oriented antiparallel to each other. One surface of the dimer, bounded by the loop between strands beta 1 and beta 2, forms a saddle-shaped groove with dimensions of approximately 24 x 23 x 12 A in cross section. Lys264, which has been shown from mutational data to be involved in DNA binding, protrudes from this surface, implicating the saddle-shaped groove as the potential DNA binding site.


==Overview==
Solution structure of the DNA binding domain of HIV-1 integrase.,Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM Biochemistry. 1995 Aug 8;34(31):9826-33. PMID:7632683<ref>PMID:7632683</ref>
The solution structure of the DNA binding domain of HIV-1 integrase (residues 220-270) has been determined by multidimensional NMR spectroscopy. The protein is a dimer in solution, and each subunit is composed of a five-stranded beta-barrel with a topology very similar to that of the SH3 domain. The dimer is formed by a stacked beta-interface comprising strands 2, 3, and 4, with the two triple-stranded antiparallel beta-sheets, one from each subunit, oriented antiparallel to each other. One surface of the dimer, bounded by the loop between strands beta 1 and beta 2, forms a saddle-shaped groove with dimensions of approximately 24 x 23 x 12 A in cross section. Lys264, which has been shown from mutational data to be involved in DNA binding, protrudes from this surface, implicating the saddle-shaped groove as the potential DNA binding site.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1IHW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHW OCA].
</div>
<div class="pdbe-citations 1ihw" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Solution structure of the DNA binding domain of HIV-1 integrase., Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM, Biochemistry. 1995 Aug 8;34(31):9826-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7632683 7632683]
*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
[[Category: Human immunodeficiency virus]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Clore, G M.]]
__TOC__
[[Category: Ernst, J A.]]
</StructureSection>
[[Category: Gronenborn, A M.]]
[[Category: Human immunodeficiency virus 1]]
[[Category: Lodi, P J.]]
[[Category: Large Structures]]
[[Category: Aid]]
[[Category: Clore GM]]
[[Category: Dna-binding protein]]
[[Category: Ernst JA]]
[[Category: Polyprotein]]
[[Category: Gronenborn AM]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:01:20 2008''
[[Category: Lodi PJ]]
Retrieved from "https://proteopedia.org/1ihw"