8b4h: Difference between revisions
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New page: '''Unreleased structure''' The entry 8b4h is ON HOLD Authors: Description: Category: Unreleased Structures |
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==IstA transposase cleaved donor complex== | |||
<StructureSection load='8b4h' size='340' side='right'caption='[[8b4h]], [[Resolution|resolution]] 3.35Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8b4h]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B4H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B4H FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.35Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b4h OCA], [https://pdbe.org/8b4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b4h RCSB], [https://www.ebi.ac.uk/pdbsum/8b4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b4h ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/TRA6_GEOSE TRA6_GEOSE] Involved in the transposition of the insertion sequence. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Transposases are ubiquitous enzymes that catalyze DNA rearrangement events with broad impacts on gene expression, genome evolution, and the spread of drug-resistance in bacteria. Here, we use biochemical and structural approaches to define the molecular determinants by which IstA, a transposase present in the widespread IS21 family of mobile elements, catalyzes efficient DNA transposition. Solution studies show that IstA engages the transposon terminal sequences to form a high-molecular weight complex and promote DNA integration. A 3.4 A resolution structure of the transposase bound to transposon ends corroborates our biochemical findings and reveals that IstA self-assembles into a highly intertwined tetramer that synapses two supercoiled terminal inverted repeats. The three-dimensional organization of the IstA*DNA cleaved donor complex reveals remarkable similarities with retroviral integrases and classic transposase systems, such as Tn7 and bacteriophage Mu, and provides insights into IS21 transposition. | |||
IS21 family transposase cleaved donor complex traps two right-handed superhelical crossings.,Spinola-Amilibia M, Araujo-Bazan L, de la Gandara A, Berger JM, Arias-Palomo E Nat Commun. 2023 Apr 22;14(1):2335. doi: 10.1038/s41467-023-38071-x. PMID:37087515<ref>PMID:37087515</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8b4h" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Geobacillus stearothermophilus]] | |||
[[Category: Large Structures]] | |||
[[Category: Araujo-Bazan L]] | |||
[[Category: Arias-Palomo E]] | |||
[[Category: Berger JM]] | |||
[[Category: Spinola-Amilibia M]] | |||
[[Category: De la Gandara A]] | |||
Latest revision as of 06:47, 24 July 2024
IstA transposase cleaved donor complex
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