6cbo: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6cbo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CBO FirstGlance]. <br>
<table><tr><td colspan='2'>[[6cbo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CBO FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DOW:(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl+2-amino-2,6-dideoxy-6-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-alpha-D-glucopyranoside'>DOW</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DOW:(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl+2-amino-2,6-dideoxy-6-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-alpha-D-glucopyranoside'>DOW</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cbo OCA], [https://pdbe.org/6cbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cbo RCSB], [https://www.ebi.ac.uk/pdbsum/6cbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cbo ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cbo OCA], [https://pdbe.org/6cbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cbo RCSB], [https://www.ebi.ac.uk/pdbsum/6cbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cbo ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/Q70KD9_MICEC Q70KD9_MICEC]  
[https://www.uniprot.org/uniprot/Q70KD9_MICEC Q70KD9_MICEC]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The aminoglycoside antibiotics, discovered as natural products in the 1940s, demonstrate a broad anti-microbial spectrum. Due to their nephrotoxic and ototoxic side effects, however, their widespread clinical usage has typically been limited to the treatment of serious infections. Neomycin B, first isolated from strains of Streptomyces in 1948, is one such drug that was approved for human use by the U. S. Food and Drug Administration in 1964. Only within the last 11 years has the biochemical pathway for its production been elaborated, however. Here we present the three-dimensional architecture of NeoB from Streptomyces fradiae, which is a pyridoxal 5'-phosphate or PLP-dependent aminotransferase that functions on two different substrates in neomycin B biosynthesis. For this investigation, four high resolution X-ray structures of NeoB were determined in various complexed states. The overall fold of NeoB is that typically observed for members of the "aspartate aminotransferase" family with the exception of an additional three-stranded anti-parallel beta-sheet that forms part of the subunit:subunit interface of the dimer. The manner in which the active site of NeoB accommodates quite different substrates has been defined by this investigation. In addition, during the course of this study, we also determined the structure of the aminotransferase GenB1 to high resolution. GenB1 functions as an aminotransferase in gentamicin biosynthesis. Taken together, the structures of NeoB and GenB1, presented here, provide the first detailed descriptions of aminotransferases that specifically function on aldehyde moieties in aminoglycoside biosynthesis. This article is protected by copyright. All rights reserved.
The Three-Dimensional Structure of NeoB: An Aminotransferase Involved in the Biosynthesis of Neomycin.,Dow GT, Thoden JB, Holden HM Protein Sci. 2018 Mar 8. doi: 10.1002/pro.3400. PMID:29516565<ref>PMID:29516565</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6cbo" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Aminotransferase 3D structures|Aminotransferase 3D structures]]
*[[Aminotransferase 3D structures|Aminotransferase 3D structures]]
== References ==
<references/>
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__TOC__
</StructureSection>
</StructureSection>

Latest revision as of 14:27, 13 March 2024

X-ray structure of GenB1 from micromonospora echinospora in complex with neamine and PLP (as the external aldimine)

6cbo, resolution 1.60Å

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