4f1r

Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginos

spinqualitylabelsall models 4f1r, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4f1r is a 1 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.201Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q51380_PSEAI Publication Abstract from PubMed The global metabolic regulator catabolite repression control (Crc) has recently been found to modulate the susceptibility to antibiotics and virulence in the opportunistic pathogen Pseudomonas aeruginosa and been suggested as a nonlethal target for novel antimicrobials. In P. aeruginosa, Crc couples with the CA motifs from the small RNA CrcZ to form a post-transcriptional regulator system and is removed from the 5'-end of the target mRNAs. In this study, we first reported the crystal structure of Crc from P. aeruginosa refined to 2.20 A. The structure showed that it consists of two halves with similar overall topology and there are 11 beta strands surrounded by 13 helices, forming a four-layered alpha/beta-sandwich. The circular dichroism spectroscopy revealed that it is thermostable in solution and shares similar characteristics to that in crystal. Comprehensive structural analysis and comparison with the homologies of Crc showed high similarity with several known nucleases and consequently may be classified into a member exodeoxyribonuclease III. However, it shows distinct substrate specificity (RNA as the preferred substrate) compared to these DNA endonucleases. Structural comparisons also revealed potential RNA recognition and binding region mainly consisting of five flexible loops. Our structure study provided the basis for the future application of Crc as a target to develop new antibiotics. (c) 2012 IUBMB Life, 65(1):50-57, 2013. Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginosa.,Wei Y, Zhang H, Gao ZQ, Xu JH, Liu QS, Dong YH IUBMB Life. 2013 Jan;65(1):50-7. doi: 10.1002/iub.1103. PMID:23281037 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA