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[[Image:1it1.jpg|left|200px]]
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{{STRUCTURE_1it1|  PDB=1it1  |  SCENE=  }}
'''Solution structures of ferrocytochrome c3 from Desulfovibrio vulgaris Miyazaki F'''


==Solution structures of ferrocytochrome c3 from Desulfovibrio vulgaris Miyazaki F==
<StructureSection load='1it1' size='340' side='right'caption='[[1it1]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1it1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._'Miyazaki_F' Desulfovibrio vulgaris str. 'Miyazaki F']. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IT1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR,  models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1it1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1it1 OCA], [https://pdbe.org/1it1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1it1 RCSB], [https://www.ebi.ac.uk/pdbsum/1it1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1it1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CYC3_NITV9 CYC3_NITV9] Participates in sulfate respiration coupled with phosphorylation by transferring electrons from the enzyme dehydrogenase to ferredoxin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/it/1it1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1it1 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Heteronuclear NMR spectroscopy was performed to determine the solution structure of (15)N-labeled ferrocytochrome c(3) from Desulfovibrio vulgaris Miyazaki F (DvMF). Although the folding of the reduced cytochrome c(3) in solution was similar to that of the oxidized one in the crystal structure, the region involving hemes 1 and 2 was different. The redox-coupled conformational change is consistent with the reported solution structure of D. vulgaris Hildenborough ferrocytochrome c(3), but is different from those of other cytochromes c(3). The former is homologous with DvMF cytochrome c(3) in amino acid sequence. Small displacements of hemes 1 and 2 relative to hemes 3 and 4 were observed. This observation is consistent with the unusual behavior of the 2(1)CH(3) signal of heme 3 reported previously. As shown by the (15)N relaxation parameters of the backbone, a region between hemes 1 and 2 has more flexibility than the other regions. The results of this work strongly suggest that the cooperative reduction of hemes 1 and 2 is based on the conformational changes of the C-13 propionate of heme 1 and the aromatic ring of Tyr43, and the interaction between His34 and His 35 through covalent and coordination bonds.


==Overview==
Redox-coupled conformational alternations in cytochrome c(3) from D. vulgaris Miyazaki F on the basis of its reduced solution structure.,Harada E, Fukuoka Y, Ohmura T, Fukunishi A, Kawai G, Fujiwara T, Akutsu H J Mol Biol. 2002 Jun 7;319(3):767-78. PMID:12054869<ref>PMID:12054869</ref>
Heteronuclear NMR spectroscopy was performed to determine the solution structure of (15)N-labeled ferrocytochrome c(3) from Desulfovibrio vulgaris Miyazaki F (DvMF). Although the folding of the reduced cytochrome c(3) in solution was similar to that of the oxidized one in the crystal structure, the region involving hemes 1 and 2 was different. The redox-coupled conformational change is consistent with the reported solution structure of D. vulgaris Hildenborough ferrocytochrome c(3), but is different from those of other cytochromes c(3). The former is homologous with DvMF cytochrome c(3) in amino acid sequence. Small displacements of hemes 1 and 2 relative to hemes 3 and 4 were observed. This observation is consistent with the unusual behavior of the 2(1)CH(3) signal of heme 3 reported previously. As shown by the (15)N relaxation parameters of the backbone, a region between hemes 1 and 2 has more flexibility than the other regions. The results of this work strongly suggest that the cooperative reduction of hemes 1 and 2 is based on the conformational changes of the C-13 propionate of heme 1 and the aromatic ring of Tyr43, and the interaction between His34 and His 35 through covalent and coordination bonds.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1IT1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IT1 OCA].
</div>
<div class="pdbe-citations 1it1" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Redox-coupled conformational alternations in cytochrome c(3) from D. vulgaris Miyazaki F on the basis of its reduced solution structure., Harada E, Fukuoka Y, Ohmura T, Fukunishi A, Kawai G, Fujiwara T, Akutsu H, J Mol Biol. 2002 Jun 7;319(3):767-78. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12054869 12054869]
*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
[[Category: Desulfovibrio vulgaris]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Akutsu, H.]]
__TOC__
[[Category: Fujiwara, T.]]
</StructureSection>
[[Category: Fukunishi, A.]]
[[Category: Desulfovibrio vulgaris str. 'Miyazaki F']]
[[Category: Fukuoka, Y.]]
[[Category: Large Structures]]
[[Category: Harada, E.]]
[[Category: Akutsu H]]
[[Category: Kawai, G.]]
[[Category: Fujiwara T]]
[[Category: Ohmura, T.]]
[[Category: Fukunishi A]]
[[Category: Electron transfer]]
[[Category: Fukuoka Y]]
[[Category: Tetraheme protein]]
[[Category: Harada E]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:22:50 2008''
[[Category: Kawai G]]
[[Category: Ohmura T]]
Retrieved from "https://proteopedia.org/1it1"