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[[Image:1itz.gif|left|200px]]
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{{STRUCTURE_1itz|  PDB=1itz  |  SCENE=  }}
'''Maize Transketolase in complex with TPP'''


==Maize Transketolase in complex with TPP==
<StructureSection load='1itz' size='340' side='right'caption='[[1itz]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1itz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ITZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ITZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1itz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1itz OCA], [https://pdbe.org/1itz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1itz RCSB], [https://www.ebi.ac.uk/pdbsum/1itz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1itz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TKTC_MAIZE TKTC_MAIZE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/it/1itz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1itz ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The gene specifying plastid transketolase (TK) of maize (Zea mays) was cloned from a cDNA library by southern blotting using a heterologous probe from sorghum (Sorghum bicolor). A recombinant fusion protein comprising thioredoxin of Escherichia coli and mature TK of maize was expressed at a high level in E. coli and cleaved with thrombin, affording plastid TK. The protein in complex with thiamine pyrophoshate was crystallized, and its structure was solved by molecular replacement. The enzyme is a C2 symmetric homodimer closely similar to the enzyme from yeast (Saccharomyces cerevisiae). Each subunit is folded into three domains. The two topologically equivalent active sites are located in the subunit interface region and resemble those of the yeast enzyme.


==Overview==
Structure and properties of an engineered transketolase from maize.,Gerhardt S, Echt S, Busch M, Freigang J, Auerbach G, Bader G, Martin WF, Bacher A, Huber R, Fischer M Plant Physiol. 2003 Aug;132(4):1941-9. PMID:12913150<ref>PMID:12913150</ref>
The gene specifying plastid transketolase (TK) of maize (Zea mays) was cloned from a cDNA library by southern blotting using a heterologous probe from sorghum (Sorghum bicolor). A recombinant fusion protein comprising thioredoxin of Escherichia coli and mature TK of maize was expressed at a high level in E. coli and cleaved with thrombin, affording plastid TK. The protein in complex with thiamine pyrophoshate was crystallized, and its structure was solved by molecular replacement. The enzyme is a C2 symmetric homodimer closely similar to the enzyme from yeast (Saccharomyces cerevisiae). Each subunit is folded into three domains. The two topologically equivalent active sites are located in the subunit interface region and resemble those of the yeast enzyme.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1ITZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ITZ OCA].
</div>
<div class="pdbe-citations 1itz" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structure and properties of an engineered transketolase from maize., Gerhardt S, Echt S, Busch M, Freigang J, Auerbach G, Bader G, Martin WF, Bacher A, Huber R, Fischer M, Plant Physiol. 2003 Aug;132(4):1941-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12913150 12913150]
*[[Transketolase 3D structures|Transketolase 3D structures]]
[[Category: Single protein]]
== References ==
[[Category: Transketolase]]
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Zea mays]]
[[Category: Zea mays]]
[[Category: Bacher, A.]]
[[Category: Bacher A]]
[[Category: Bader, G.]]
[[Category: Bader G]]
[[Category: Busch, M.]]
[[Category: Busch M]]
[[Category: Echt, S.]]
[[Category: Echt S]]
[[Category: Fischer, M.]]
[[Category: Fischer M]]
[[Category: Freigang, J.]]
[[Category: Freigang J]]
[[Category: Gerhardt, S.]]
[[Category: Gerhardt S]]
[[Category: Huber, R.]]
[[Category: Huber R]]
[[Category: Calvin cycle]]
[[Category: Cofactor]]
[[Category: Plant]]
[[Category: Thiamine pyrophosphate]]
[[Category: Transketolase]]
[[Category: Zea may]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:24:39 2008''

Latest revision as of 23:36, 27 December 2023

Maize Transketolase in complex with TPP

1itz, resolution 2.30Å

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