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New page: left|200px<br /> <applet load="2iwv" size="450" color="white" frame="true" align="right" spinBox="true" caption="2iwv, resolution 2.30Å" /> '''STRUCTURE OF THE MO...
 
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[[Image:2iwv.gif|left|200px]]<br />
<applet load="2iwv" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2iwv, resolution 2.30&Aring;" />
'''STRUCTURE OF THE MONOMERIC OUTER MEMBRANE PORIN OMPG IN THE OPEN AND CLOSED CONFORMATION'''<br />


==About this Structure==
==Structure of the monomeric outer membrane porin OmpG in the open and closed conformation==
2IWV is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with CA, LDA and TAM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IWV OCA]].  
<StructureSection load='2iwv' size='340' side='right'caption='[[2iwv]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
[[Category: Escherichia coli]]
== Structural highlights ==
[[Category: Single protein]]
<table><tr><td colspan='2'>[[2iwv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IWV FirstGlance]. <br>
[[Category: Goswami, P.]]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
[[Category: Kuehlbrandt, W.]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
[[Category: Vinothkumar, K.R.]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iwv OCA], [https://pdbe.org/2iwv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iwv RCSB], [https://www.ebi.ac.uk/pdbsum/2iwv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iwv ProSAT]</span></td></tr>
[[Category: Yildiz, O.]]
</table>
[[Category: CA]]
== Function ==
[[Category: LDA]]
[https://www.uniprot.org/uniprot/OMPG_ECOLI OMPG_ECOLI] Forms channels functionally larger than those of classical porins.<ref>PMID:11758943</ref>  May act as a regulator of the RCS-phosphorelay signal transduction pathway.<ref>PMID:11758943</ref>
[[Category: TAM]]
<div style="background-color:#fffaf0;">
[[Category: ion channel]]
== Publication Abstract from PubMed ==
[[Category: ion transport]]
OmpG, a monomeric pore-forming protein from Escherichia coli outer membranes, was refolded from inclusion bodies and crystallized in two different conformations. The OmpG channel is a 14-stranded beta-barrel, with short periplasmic turns and seven extracellular loops. Crystals grown at neutral pH show the channel in the open state at 2.3 A resolution. In the 2.7 A structure of crystals grown at pH 5.6, the pore is blocked by loop 6, which folds across the channel. The rearrangement of loop 6 appears to be triggered by a pair of histidine residues, which repel one another at acidic pH, resulting in the breakage of neighbouring H-bonds and a lengthening of loop 6 from 10 to 17 residues. A total of 151 ordered LDAO detergent molecules were found in the 2.3 A structure, mostly on the hydrophobic outer surface of OmpG, mimicking the outer membrane lipid bilayer, with three LDAO molecules in the open pore. In the 2.7 A structure, OmpG binds one OG and one glucose molecule as sugar substrates in the closed pore.
[[Category: membrane]]
[[Category: outer membrane]]
[[Category: porin]]
[[Category: transmembrane]]
[[Category: transport]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 18:11:37 2007''
Structure of the monomeric outer-membrane porin OmpG in the open and closed conformation.,Yildiz O, Vinothkumar KR, Goswami P, Kuhlbrandt W EMBO J. 2006 Aug 9;25(15):3702-13. Epub 2006 Aug 3. PMID:16888630<ref>PMID:16888630</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2iwv" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Porin 3D structures|Porin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Goswami P]]
[[Category: Kuehlbrandt W]]
[[Category: Vinothkumar KR]]
[[Category: Yildiz O]]
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