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[[Image:1j19.gif|left|200px]]
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{{STRUCTURE_1j19|  PDB=1j19  |  SCENE=  }}
'''Crystal structure of the radxin FERM domain complexed with the ICAM-2 cytoplasmic peptide'''


==Crystal structure of the radxin FERM domain complexed with the ICAM-2 cytoplasmic peptide==
<StructureSection load='1j19' size='340' side='right'caption='[[1j19]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1j19]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J19 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j19 OCA], [https://pdbe.org/1j19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j19 RCSB], [https://www.ebi.ac.uk/pdbsum/1j19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j19 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RADI_MOUSE RADI_MOUSE] Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j1/1j19_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j19 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
ERM (ezrin/radixin/moesin) proteins recognize the cytoplasmic domains of adhesion molecules in the formation of the membrane-associated cytoskeleton. Here we report the crystal structure of the radixin FERM (4.1 and ERM) domain complexed with the ICAM-2 cytoplasmic peptide. The non-polar region of the ICAM-2 peptide contains the RxxTYxVxxA sequence motif to form a beta-strand followed by a short 3(10)-helix. It binds the groove of the phosphotyrosine-binding (PTB)-like subdomain C mediated by a beta-beta association and several side-chain interactions. The binding mode of the ICAM-2 peptide to the FERM domain is distinct from that of the NPxY motif-containing peptide binding to the canonical PTB domain. Mutation analyses based on the crystal structure reveal the determinant elements of recognition and provide the first insights into the physical link between adhesion molecules and ERM proteins.


==Overview==
Structural basis of adhesion-molecule recognition by ERM proteins revealed by the crystal structure of the radixin-ICAM-2 complex.,Hamada K, Shimizu T, Yonemura S, Tsukita S, Tsukita S, Hakoshima T EMBO J. 2003 Feb 3;22(3):502-14. PMID:12554651<ref>PMID:12554651</ref>
ERM (ezrin/radixin/moesin) proteins recognize the cytoplasmic domains of adhesion molecules in the formation of the membrane-associated cytoskeleton. Here we report the crystal structure of the radixin FERM (4.1 and ERM) domain complexed with the ICAM-2 cytoplasmic peptide. The non-polar region of the ICAM-2 peptide contains the RxxTYxVxxA sequence motif to form a beta-strand followed by a short 3(10)-helix. It binds the groove of the phosphotyrosine-binding (PTB)-like subdomain C mediated by a beta-beta association and several side-chain interactions. The binding mode of the ICAM-2 peptide to the FERM domain is distinct from that of the NPxY motif-containing peptide binding to the canonical PTB domain. Mutation analyses based on the crystal structure reveal the determinant elements of recognition and provide the first insights into the physical link between adhesion molecules and ERM proteins.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1J19 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J19 OCA].
</div>
<div class="pdbe-citations 1j19" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structural basis of adhesion-molecule recognition by ERM proteins revealed by the crystal structure of the radixin-ICAM-2 complex., Hamada K, Shimizu T, Yonemura S, Tsukita S, Tsukita S, Hakoshima T, EMBO J. 2003 Feb 3;22(3):502-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12554651 12554651]
*[[Radixin|Radixin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Hakoshima T]]
[[Category: Hakoshima, T.]]
[[Category: Hamada K]]
[[Category: Hamada, K.]]
[[Category: Shimizu T]]
[[Category: Shimizu, T.]]
[[Category: Tsukita S]]
[[Category: Tsukita, S.]]
[[Category: Yonemura S]]
[[Category: Yonemura, S.]]
[[Category: Protein-peptide complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:40:39 2008''
Retrieved from "https://proteopedia.org/1j19"