8fcj: Difference between revisions
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The | ==Cryo-EM structure of Cascade-DNA (P23) complex in type I-B CAST system== | ||
<StructureSection load='8fcj' size='340' side='right'caption='[[8fcj]], [[Resolution|resolution]] 2.83Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8fcj]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._'Peltigera_membranacea_cyanobiont'_210A Nostoc sp. 'Peltigera membranacea cyanobiont' 210A] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FCJ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.83Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fcj OCA], [https://pdbe.org/8fcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fcj RCSB], [https://www.ebi.ac.uk/pdbsum/8fcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fcj ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A235IG00_9NOSO A0A235IG00_9NOSO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Tn7-like transposons have co-opted CRISPR-Cas systems to facilitate the movement of their own DNA. These CRISPR-associated transposons (CASTs) are promising tools for programmable gene knockin. A key feature of CASTs is their ability to recruit Tn7-like transposons to nuclease-deficient CRISPR effectors. However, how Tn7-like transposons are recruited by diverse CRISPR effectors remains poorly understood. Here, we present the cryo-EM structure of a recruitment complex comprising the Cascade complex, TniQ, TnsC, and the target DNA in the type I-B CAST from Peltigera membranacea cyanobiont 210A. Target DNA recognition by Cascade induces conformational changes in Cas6 and primes TniQ recruitment through its C-terminal domain. The N-terminal domain of TniQ is bound to the seam region of the TnsC spiral heptamer. Our findings provide insights into the diverse mechanisms for the recruitment of Tn7-like transposons to CRISPR effectors and will aid in the development of CASTs as gene knockin tools. | |||
Molecular mechanism for Tn7-like transposon recruitment by a type I-B CRISPR effector.,Wang S, Gabel C, Siddique R, Klose T, Chang L Cell. 2023 Sep 14;186(19):4204-4215.e19. doi: 10.1016/j.cell.2023.07.010. Epub , 2023 Aug 8. PMID:37557170<ref>PMID:37557170</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8fcj" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Nostoc sp. 'Peltigera membranacea cyanobiont' 210A]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Chang L]] | |||
[[Category: Wang S]] | |||
Latest revision as of 06:05, 28 May 2025
Cryo-EM structure of Cascade-DNA (P23) complex in type I-B CAST system
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