7ot9: Difference between revisions

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== Structural highlights ==

<table><tr><td colspan='2'>~~[[7ot9]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OT9 FirstGlance]. <br>

<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OT9 FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ot9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ot9 OCA], [https://pdbe.org/7ot9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ot9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ot9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ot9 ProSAT]</span></td></tr>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8Å</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ot9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ot9 OCA], [https://pdbe.org/7ot9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ot9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ot9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ot9 ProSAT]</span></td></tr>

</table>

~~== Function ==~~

~~[https://www.uniprot.org/uniprot/YDIK_ECOLI YDIK_ECOLI]~~

~~<div style="background-color:#fffaf0;">~~

~~== Publication Abstract from PubMed ==~~

Bacteria utilize small extracellular molecules to communicate in order to collectively coordinate their behaviors in response to the population density. Autoinducer-2 (AI-2), a universal molecule for both intra- and inter-species communication, is involved in the regulation of biofilm formation, virulence, motility, chemotaxis, and antibiotic resistance. While many studies have been devoted to understanding the biosynthesis and sensing of AI-2, very little information is available on its export. The protein TqsA from Escherichia coli, which belongs to the AI-2 exporter superfamily, has been shown to export AI-2. Here, we report the cryogenic electron microscopic structures of two AI-2 exporters (TqsA and YdiK) from E. coli at 3.35 A and 2.80 A resolutions, respectively. Our structures suggest that the AI-2 exporter exists as a homo-pentameric complex. In silico molecular docking and native mass spectrometry experiments were employed to demonstrate the interaction between AI-2 and TqsA, and the results highlight the functional importance of two helical hairpins in substrate binding. We propose that each monomer works as an independent functional unit utilizing an elevator-type transport mechanism.

Cryo-EM structures of pentameric autoinducer-2 exporter from Escherichia coli reveal its transport mechanism.,Khera R, Mehdipour AR, Bolla JR, Kahnt J, Welsch S, Ermler U, Muenke C, Robinson CV, Hummer G, Xie H, Michel H EMBO J. 2022 Jun 14:e109990. doi: 10.15252/embj.2021109990. PMID:35698912<ref>PMID:35698912</ref>

~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~

~~</div>~~

~~<div class="pdbe-citations 7ot9" style="background-color:#fffaf0;"></div>~~

~~== References ==~~

~~<references/>~~

__TOC__

</StructureSection>

~~[[Category: Escherichia coli K-12]]~~

[[Category: Large Structures]]

[[Category: Khera R]]

[[Category: Michel H]]

[[Category: Xie H]]

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@@ Line 3: / Line 3: @@
 <StructureSection load='7ot9' size='340' side='right'caption='[[7ot9]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7ot9]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OT9 FirstGlance]. <br>
+<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OT9 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ot9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ot9 OCA], [https://pdbe.org/7ot9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ot9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ot9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ot9 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ot9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ot9 OCA], [https://pdbe.org/7ot9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ot9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ot9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ot9 ProSAT]</span></td></tr>
 </table>
-== Function ==
-[https://www.uniprot.org/uniprot/YDIK_ECOLI YDIK_ECOLI]
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Bacteria utilize small extracellular molecules to communicate in order to collectively coordinate their behaviors in response to the population density. Autoinducer-2 (AI-2), a universal molecule for both intra- and inter-species communication, is involved in the regulation of biofilm formation, virulence, motility, chemotaxis, and antibiotic resistance. While many studies have been devoted to understanding the biosynthesis and sensing of AI-2, very little information is available on its export. The protein TqsA from Escherichia coli, which belongs to the AI-2 exporter superfamily, has been shown to export AI-2. Here, we report the cryogenic electron microscopic structures of two AI-2 exporters (TqsA and YdiK) from E. coli at 3.35 A and 2.80 A resolutions, respectively. Our structures suggest that the AI-2 exporter exists as a homo-pentameric complex. In silico molecular docking and native mass spectrometry experiments were employed to demonstrate the interaction between AI-2 and TqsA, and the results highlight the functional importance of two helical hairpins in substrate binding. We propose that each monomer works as an independent functional unit utilizing an elevator-type transport mechanism.
-Cryo-EM structures of pentameric autoinducer-2 exporter from Escherichia coli reveal its transport mechanism.,Khera R, Mehdipour AR, Bolla JR, Kahnt J, Welsch S, Ermler U, Muenke C, Robinson CV, Hummer G, Xie H, Michel H EMBO J. 2022 Jun 14:e109990. doi: 10.15252/embj.2021109990. PMID:35698912<ref>PMID:35698912</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 7ot9" style="background-color:#fffaf0;"></div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Escherichia coli K-12]]
 [[Category: Large Structures]]
 [[Category: Khera R]]
 [[Category: Michel H]]
 [[Category: Xie H]]