8byp: Difference between revisions
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New page: '''Unreleased structure''' The entry 8byp is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Botulinum neurotoxin serotype X in complex with NTNH/X== | |||
<StructureSection load='8byp' size='340' side='right'caption='[[8byp]], [[Resolution|resolution]] 3.12Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8byp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BYP FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.12Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8byp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8byp OCA], [https://pdbe.org/8byp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8byp RCSB], [https://www.ebi.ac.uk/pdbsum/8byp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8byp ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Botulinum neurotoxins (BoNTs) are the most potent toxins known and are used to treat an increasing number of medical disorders. All BoNTs are naturally co-expressed with a protective partner protein (NTNH) with which they form a 300 kDa complex, to resist acidic and proteolytic attack from the digestive tract. We have previously identified a new botulinum neurotoxin serotype, BoNT/X, that has unique and therapeutically attractive properties. We present the cryo-EM structure of the BoNT/X-NTNH/X complex at 3.1 A resolution. Unexpectedly, the BoNT/X complex is stable and protease resistant at both neutral and acidic pH and disassembles only in alkaline conditions. Using the stabilizing effect of NTNH, we isolated BoNT/X and showed that it has very low potency both in vitro and in vivo . Given the high catalytic activity and translocation efficacy of BoNT/X, low activity of the full toxin is likely due to the receptor-binding domain, which presents weak ganglioside binding and exposed hydrophobic surfaces. | |||
Structure and activity of botulinum neurotoxin X.,Martinez-Carranza M, Skerlova J, Lee PG, Zhang J, Burgin D, Elliott M, Philippe J, Donald S, Hornby F, Henriksson L, Masuyer G, Beard M, Dong M, Stenmark P bioRxiv [Preprint]. 2023 Jan 11:2023.01.11.523524. doi: , 10.1101/2023.01.11.523524. PMID:36712025<ref>PMID:36712025</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8byp" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Clostridium botulinum]] | |||
[[Category: Large Structures]] | |||
[[Category: Martinez-Carranza M]] | |||
[[Category: Skerlova J]] | |||
[[Category: Stenmark P]] | |||
Latest revision as of 09:30, 17 October 2024
Botulinum neurotoxin serotype X in complex with NTNH/X
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