8hrg: Difference between revisions
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New page: '''Unreleased structure''' The entry 8hrg is ON HOLD Authors: Description: Category: Unreleased Structures |
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The | ==Tail tube of DT57C bacteriophage in the full state== | ||
<StructureSection load='8hrg' size='340' side='right'caption='[[8hrg]], [[Resolution|resolution]] 4.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8hrg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tequintavirus Tequintavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HRG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HRG FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hrg OCA], [https://pdbe.org/8hrg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hrg RCSB], [https://www.ebi.ac.uk/pdbsum/8hrg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hrg ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0A7RSI5_9CAUD A0A0A7RSI5_9CAUD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The T5 family of viruses are tailed bacteriophages characterized by a long non-contractile tail. The bacteriophage DT57C is closely related to the paradigmal T5 phage, though it recognizes a different receptor (BtuB) and features highly divergent lateral tail fibers (LTF). Considerable portions of T5-like phages remain structurally uncharacterized. Here, we present the structure of DT57C determined by cryo-EM, and an atomic model of the virus, which was further explored using all-atom molecular dynamics simulations. The structure revealed a unique way of LTF attachment assisted by a dodecameric collar protein LtfC, and an unusual composition of the phage neck constructed of three protein rings. The tape measure protein (TMP) is organized within the tail tube in a three-stranded parallel alpha-helical coiled coil which makes direct contact with the genomic DNA. The presence of the C-terminal fragment of the TMP that remains within the tail tip suggests that the tail tip complex returns to its original state after DNA ejection. Our results provide a complete atomic structure of a T5-like phage, provide insights into the process of DNA ejection as well as a structural basis for the design of engineered phages and future mechanistic studies. | |||
Nearly complete structure of bacteriophage DT57C reveals architecture of head-to-tail interface and lateral tail fibers.,Ayala R, Moiseenko AV, Chen TH, Kulikov EE, Golomidova AK, Orekhov PS, Street MA, Sokolova OS, Letarov AV, Wolf M Nat Commun. 2023 Dec 11;14(1):8205. doi: 10.1038/s41467-023-43824-9. PMID:38081816<ref>PMID:38081816</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8hrg" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Tequintavirus]] | |||
[[Category: Ayala R]] | |||
[[Category: Chen TH]] | |||
[[Category: Golomidova AK]] | |||
[[Category: Kulikov EE]] | |||
[[Category: Letarov AV]] | |||
[[Category: Moiseenko AV]] | |||
[[Category: Orekhov PS]] | |||
[[Category: Sokolova OS]] | |||
[[Category: Street MA]] | |||
[[Category: Wolf M]] | |||
