8h6e: Difference between revisions
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==Cryo-EM structure of human exon-defined spliceosome in the late pre-B state.== | |||
<StructureSection load='8h6e' size='340' side='right'caption='[[8h6e]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8h6e]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8H6E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8H6E FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=M7M:N,N,7-TRIMETHYLGUANOSINE+5-(TRIHYDROGEN+DIPHOSPHATE)'>M7M</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8h6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8h6e OCA], [https://pdbe.org/8h6e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8h6e RCSB], [https://www.ebi.ac.uk/pdbsum/8h6e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8h6e ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Spliceosome is often assembled across an exon and undergoes rearrangement to span a neighboring intron. Most states of the intron-defined spliceosome have been structurally characterized. However, the structure of a fully assembled exon-defined spliceosome remains at large. During spliceosome assembly, the pre-catalytic state (B complex) is converted from its precursor (pre-B complex). Here we report atomic structures of the exon-defined human spliceosome in four sequential states: mature pre-B, late pre-B, early B, and mature B. In the previously unknown late pre-B state, U1 snRNP is already released but the remaining proteins are still in the pre-B state; unexpectedly, the RNAs are in the B state, with U6 snRNA forming a duplex with 5'-splice site and U5 snRNA recognizing the 3'-end of the exon. In the early and mature B complexes, the B-specific factors are stepwise recruited and specifically recognize the exon 3'-region. Our study reveals key insights into the assembly of the exon-defined spliceosomes and identifies mechanistic steps of the pre-B-to-B transition. | |||
Structural insights into human exon-defined spliceosome prior to activation.,Zhang W, Zhang X, Zhan X, Bai R, Lei J, Yan C, Shi Y Cell Res. 2024 Jun;34(6):428-439. doi: 10.1038/s41422-024-00949-w. Epub 2024 Apr , 24. PMID:38658629<ref>PMID:38658629</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8h6e" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Bai R]] | |||
[[Category: Lei J]] | |||
[[Category: Shi Y]] | |||
[[Category: Yan C]] | |||
[[Category: Zhan X]] | |||
[[Category: Zhang W]] | |||
[[Category: Zhang X]] | |||
