1iap: Difference between revisions

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New page: left|200px<br /> <applet load="1iap" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iap, resolution 1.90Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:1iap.gif|left|200px]]<br />
<applet load="1iap" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1iap, resolution 1.90&Aring;" />
'''CRYSTAL STRUCTURE OF P115RHOGEF RGRGS DOMAIN'''<br />


==Overview==
==CRYSTAL STRUCTURE OF P115RHOGEF RGRGS DOMAIN==
p115RhoGEF, a guanine nucleotide exchange factor for Rho GTPase, is also a, GTPase activating protein (GAP) for G(12) and G(13) heterotrimeric G alpha, subunits. Near its N-terminus, p115RhoGEF contains a domain (rgRGS) with, remote sequence identity to RGS (regulators of G protein signaling), domains. The rgRGS domain is necessary but not sufficient for the GAP, activity of p115RhoGEF. The 1.9 A resolution crystal structure of the, rgRGS domain shows structural similarity to RGS domains but possesses a, C-terminal extension that folds into a layer of helices that pack against, the hydrophobic core of the domain. Mutagenesis experiments show that, rgRGS may form interactions with G alpha(13) that are analogous to those, in complexes of RGS proteins with their G alpha substrates.
<StructureSection load='1iap' size='340' side='right'caption='[[1iap]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1iap]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IAP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iap OCA], [https://pdbe.org/1iap PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iap RCSB], [https://www.ebi.ac.uk/pdbsum/1iap PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iap ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ARHG1_HUMAN ARHG1_HUMAN] Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.<ref>PMID:8810315</ref> <ref>PMID:9641915</ref> <ref>PMID:9641916</ref> <ref>PMID:20098430</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/1iap_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iap ConSurf].
<div style="clear:both"></div>


==Disease==
==See Also==
Known disease associated with this structure: Slowed nerve conduction velocity, AD OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=608136 608136]]
*[[Rho guanine nucleotide exchange factor 3D structures|Rho guanine nucleotide exchange factor 3D structures]]
 
== References ==
==About this Structure==
<references/>
1IAP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IAP OCA].
__TOC__
 
</StructureSection>
==Reference==
Structure of the rgRGS domain of p115RhoGEF., Chen Z, Wells CD, Sternweis PC, Sprang SR, Nat Struct Biol. 2001 Sep;8(9):805-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11524686 11524686]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Chen, Z.]]
[[Category: Chen Z]]
[[Category: Sprang, S.R.]]
[[Category: Sprang SR]]
[[Category: p115]]
[[Category: rgrgs]]
[[Category: rgs]]
[[Category: rhogef]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:28:07 2007''

Latest revision as of 07:33, 7 February 2024

CRYSTAL STRUCTURE OF P115RHOGEF RGRGS DOMAIN

1iap, resolution 1.90Å

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