8i2r: Difference between revisions
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The | ==Beijerinckia indica beta-fructosyltransferase variant H395R/F473Y in complex with fructose== | ||
<StructureSection load='8i2r' size='340' side='right'caption='[[8i2r]], [[Resolution|resolution]] 1.36Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8i2r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Beijerinckia_indica_subsp._indica Beijerinckia indica subsp. indica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I2R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I2R FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.36Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDF:BETA-D-FRUCTOPYRANOSE'>BDF</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i2r OCA], [https://pdbe.org/8i2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i2r RCSB], [https://www.ebi.ac.uk/pdbsum/8i2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i2r ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B2IF78_BEII9 B2IF78_BEII9] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The trisaccharide 1-kestose, a major constituent of fructooligosaccharide, has strong prebiotic effects. We used high-performance liquid chromatography and 1H nuclear magnetic resonance spectroscopy to show that BiBftA, a beta-fructosyltransferase belonging to glycoside hydrolase family 68, from Beijerinckia indica subsp. indica catalyzes transfructosylation of sucrose to produce mostly 1-kestose and levan polysaccharides. We substituted His395 and Phe473 in BiBftA with Arg and Tyr, respectively, and analyzed the reactions of the mutant enzymes with 180 g/L sucrose. The ratio of the molar concentrations of glucose and 1-kestose in the reaction mixture with wild-type BiBftA was 100:8.1, whereas that in the reaction mixture with the variant H395R/F473Y was 100:45.5, indicating that H395R/F473Y predominantly accumulated 1-kestose from sucrose. The X-ray crystal structure of H395R/F473Y suggests that its catalytic pocket is unfavorable for binding of sucrose while favorable for transfructosylation. | |||
Characterization and alteration of product specificity of Beijerinckia indica subsp. indica beta-fructosyltransferase.,Li D, Miyasaka Y, Kubota A, Kozono T, Kitano Y, Sasaki N, Fujii T, Tochio T, Kadota Y, Nishikawa A, Tonozuka T Biosci Biotechnol Biochem. 2023 Aug 23;87(9):981-990. doi: 10.1093/bbb/zbad074. PMID:37280168<ref>PMID:37280168</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Tonozuka | <div class="pdbe-citations 8i2r" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Beijerinckia indica subsp. indica]] | |||
[[Category: Large Structures]] | |||
[[Category: Tonozuka T]] | |||
