4oqq

Structure of the effector-binding domain of deoxyribonucleoside regulator DeoR from Bacillus subtilis

spinqualitylabelsall models 4oqq, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4oqq is a 2 chain structure with sequence from Bacillus subtilis subsp. subtilis str. 168. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: BCN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function DEOR_BACSU Negative regulator of the dra-nupC-pdp operon. DeoR binds cooperatively to the operator DNA, which consists of a palindrome and a direct repeat sequence located 3' to the palindrome. Publication Abstract from PubMed Deoxyribonucleoside regulator DeoR from Bacillus subtilis negatively regulates expression of enzymes involved in the catabolism of deoxyribonucleosides and deoxyribose. The DeoR protein is homologous to the sorbitol operon regulator family of metabolic regulators and comprises an N-terminal DNA-binding domain and a C-terminal effector-binding domain. We have determined the crystal structure of the effector-binding domain of DeoR (C-DeoR) in free form and in covalent complex with its effector deoxyribose-5-phosphate (dR5P). This is the first case of a covalently attached effector molecule captured in the structure of a bacterial transcriptional regulator. The dR5P molecule is attached through a Schiff base linkage to residue Lys141. The crucial role of Lys141 in effector binding was confirmed by mutational analysis and mass spectrometry of Schiff base adducts formed in solution. Structural analyses of the free and effector-bound C-DeoR structures provided a structural explanation for mechanism of DeoR function as a molecular switch. This article is protected by copyright. All rights reserved. Structure of the effector-binding domain of deoxyribonucleoside regulator DeoR from Bacillus subtilis.,Skerlova J, Fabry M, Hubalek M, Otwinowski Z, Rezacova P FEBS J. 2014 May 23. doi: 10.1111/febs.12856. PMID:24863636 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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