8g9s: Difference between revisions

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'''Unreleased structure'''


The entry 8g9s is ON HOLD
==Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications==
<StructureSection load='8g9s' size='340' side='right'caption='[[8g9s]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8g9s]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_lactamica Neisseria lactamica] and [https://en.wikipedia.org/wiki/Rhodobacter_phage_RcNL1 Rhodobacter phage RcNL1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8G9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8G9S FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8g9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8g9s OCA], [https://pdbe.org/8g9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8g9s RCSB], [https://www.ebi.ac.uk/pdbsum/8g9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8g9s ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D0W8X6_NEILA D0W8X6_NEILA]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Type I CRISPR-Cas systems utilize the RNA-guided Cascade complex to identify matching DNA targets and the nuclease-helicase Cas3 to degrade them. Among the seven subtypes, type I-C is compact in size and highly active in creating large-sized genome deletions in human cells. Here, we use four cryoelectron microscopy snapshots to define its RNA-guided DNA binding and cleavage mechanisms in high resolution. The non-target DNA strand (NTS) is accommodated by I-C Cascade in a continuous binding groove along the juxtaposed Cas11 subunits. Binding of Cas3 further traps a flexible bulge in NTS, enabling NTS nicking. We identified two anti-CRISPR proteins AcrIC8 and AcrIC9 that strongly inhibit Neisseria lactamica I-C function. Structural analysis showed that AcrIC8 inhibits PAM recognition through allosteric inhibition, whereas AcrIC9 achieves so through direct competition. Both Acrs potently inhibit I-C-mediated genome editing and transcriptional modulation in human cells, providing the first off-switches for type I CRISPR eukaryotic genome engineering.


Authors:  
Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications.,Hu C, Myers MT, Zhou X, Hou Z, Lozen ML, Nam KH, Zhang Y, Ke A Mol Cell. 2024 Feb 1;84(3):463-475.e5. doi: 10.1016/j.molcel.2023.12.034. Epub , 2024 Jan 18. PMID:38242128<ref>PMID:38242128</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 8g9s" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Neisseria lactamica]]
[[Category: Rhodobacter phage RcNL1]]
[[Category: Hu C]]
[[Category: Ke A]]
[[Category: Nam KH]]
Retrieved from "https://proteopedia.org/8g9s"