8fne: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
Line 4: Line 4:
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[8fne]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Pseudomonas_phage_PhiPA3 Pseudomonas phage PhiPA3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FNE FirstGlance]. <br>
<table><tr><td colspan='2'>[[8fne]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Pseudomonas_phage_PhiPA3 Pseudomonas phage PhiPA3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FNE FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fne OCA], [https://pdbe.org/8fne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fne RCSB], [https://www.ebi.ac.uk/pdbsum/8fne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fne ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fne OCA], [https://pdbe.org/8fne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fne RCSB], [https://www.ebi.ac.uk/pdbsum/8fne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fne ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/CHMA_BPPA3 CHMA_BPPA3] Self-assembles to forms a proteinaceous shell that encloses the viral DNA and compartmentalizes proteins and DNA during viral infection (PubMed:28813669). This micrometer-scale compartment contains narrow pores and is the site of viral replication, with the proteins involved in DNA replication localized inside (By similarity). Provides a surface for docking of capsids during packaging (PubMed:28813669). Probably protects the viral genome against host defenses (By similarity).[UniProtKB:B3FIW8]<ref>PMID:28813669</ref>  
[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/CHMA_BPPA3 CHMA_BPPA3] Self-assembles to forms a proteinaceous shell that encloses the viral DNA and compartmentalizes proteins and DNA during viral infection (PubMed:28813669). This micrometer-scale compartment contains narrow pores and is the site of viral replication, with the proteins involved in DNA replication localized inside (By similarity). Provides a surface for docking of capsids during packaging (PubMed:28813669). Probably protects the viral genome against host defenses (By similarity).[UniProtKB:B3FIW8]<ref>PMID:28813669</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
To protect themselves from host attack, numerous jumbo bacteriophages establish a phage nucleus-a micron-scale, proteinaceous structure encompassing the replicating phage DNA. Bacteriophage and host proteins associated with replication and transcription are concentrated inside the phage nucleus while other phage and host proteins are excluded, including CRISPR-Cas and restriction endonuclease host defense systems. Here, we show that nucleus fragments isolated from varphiPA3 infected Pseudomonas aeruginosa form a 2-dimensional lattice, having p2 or p4 symmetry. We further demonstrate that recombinantly purified primary Phage Nuclear Enclosure (PhuN) protein spontaneously assembles into similar 2D sheets with p2 and p4 symmetry. We resolve the dominant p2 symmetric state to 3.9 A by cryo-EM. Our structure reveals a two-domain core, organized into quasi-symmetric tetramers. Flexible loops and termini mediate adaptable inter-tetramer contacts that drive subunit assembly into a lattice and enable the adoption of different symmetric states. While the interfaces between subunits are mostly well packed, two are open, forming channels that likely have functional implications for the transport of proteins, mRNA, and small molecules.


The varphiPA3 phage nucleus is enclosed by a self-assembling 2D crystalline lattice.,Nieweglowska ES, Brilot AF, Mendez-Moran M, Kokontis C, Baek M, Li J, Cheng Y, Baker D, Bondy-Denomy J, Agard DA Nat Commun. 2023 Feb 18;14(1):927. doi: 10.1038/s41467-023-36526-9. PMID:36807264<ref>PMID:36807264</ref>
==See Also==
 
*[[Maltose-binding protein 3D structures|Maltose-binding protein 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8fne" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
Retrieved from "https://proteopedia.org/8fne"