4rjf: Difference between revisions

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== Structural highlights ==

<table><tr><td colspan='2'>[[4rjf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RJF FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjf OCA], [https://pdbe.org/4rjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rjf RCSB], [https://www.ebi.ac.uk/pdbsum/4rjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rjf ProSAT]</span></td></tr>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.0072Å</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjf OCA], [https://pdbe.org/4rjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rjf RCSB], [https://www.ebi.ac.uk/pdbsum/4rjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rjf ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/~~CDN1A_HUMAN CDN1A_HUMAN~~] ~~May be~~ the ~~important intermediate~~ by ~~which p53/TP53 mediates its~~ role ~~as an inhibitor of cellular proliferation~~ in response to DNA damage. ~~Binds~~ to and ~~inhibits cyclin~~-~~dependent kinase activity, preventing phosphorylation~~ of ~~critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions~~ in ~~the nuclear localization and assembly of cyclin D~~-~~CDK4 complex~~ and ~~promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits~~ the ~~kinase activity of the cyclin D-CDK4 complex~~.<ref>PMID:~~8242751~~</ref> <ref>PMID:~~9106657~~</ref>

[https://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>

== Publication Abstract from PubMed ==

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 == Structural highlights ==
 <table><tr><td colspan='2'>[[4rjf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RJF FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjf OCA], [https://pdbe.org/4rjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rjf RCSB], [https://www.ebi.ac.uk/pdbsum/4rjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rjf ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.0072&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjf OCA], [https://pdbe.org/4rjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rjf RCSB], [https://www.ebi.ac.uk/pdbsum/4rjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rjf ProSAT]</span></td></tr>
 </table>
 == Function ==
-[https://www.uniprot.org/uniprot/CDN1A_HUMAN CDN1A_HUMAN] May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.<ref>PMID:8242751</ref> <ref>PMID:9106657</ref>
+[https://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==