8cm4: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
==W-formate dehydrogenase C872A from Desulfovibrio vulgaris - exposed to oxygen== | |||
<StructureSection load='8cm4' size='340' side='right'caption='[[8cm4]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8cm4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CM4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CM4 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=H2S:HYDROSULFURIC+ACID'>H2S</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=W:TUNGSTEN+ION'>W</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cm4 OCA], [https://pdbe.org/8cm4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cm4 RCSB], [https://www.ebi.ac.uk/pdbsum/8cm4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cm4 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q72EJ1_NITV2 Q72EJ1_NITV2] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Metal-dependent formate dehydrogenases reduce CO(2) with high efficiency and selectivity, but are usually very oxygen sensitive. An exception is Desulfovibrio vulgaris W/Sec-FdhAB, which can be handled aerobically, but the basis for this oxygen tolerance was unknown. Here we show that FdhAB activity is controlled by a redox switch based on an allosteric disulfide bond. When this bond is closed, the enzyme is in an oxygen-tolerant resting state presenting almost no catalytic activity and very low formate affinity. Opening this bond triggers large conformational changes that propagate to the active site, resulting in high activity and high formate affinity, but also higher oxygen sensitivity. We present the structure of activated FdhAB and show that activity loss is associated with partial loss of the metal sulfido ligand. The redox switch mechanism is reversible in vivo and prevents enzyme reduction by physiological formate levels, conferring a fitness advantage during O(2) exposure. | |||
An allosteric redox switch involved in oxygen protection in a CO(2) reductase.,Oliveira AR, Mota C, Vilela-Alves G, Manuel RR, Pedrosa N, Fourmond V, Klymanska K, Leger C, Guigliarelli B, Romao MJ, Cardoso Pereira IA Nat Chem Biol. 2024 Jan;20(1):111-119. doi: 10.1038/s41589-023-01484-2. Epub 2023 , Nov 20. PMID:37985883<ref>PMID:37985883</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8cm4" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Desulfovibrio vulgaris str. Hildenborough]] | |||
[[Category: Large Structures]] | |||
[[Category: Klymanska K]] | |||
[[Category: Manuel RR]] | |||
[[Category: Mota C]] | |||
[[Category: Oliveira AR]] | |||
[[Category: Pereira IC]] | |||
[[Category: Romao MJ]] | |||
[[Category: Vilela-Alves G]] | |||
Latest revision as of 19:37, 8 September 2026
W-formate dehydrogenase C872A from Desulfovibrio vulgaris - exposed to oxygen
| ||||||||||||
