8i74: Difference between revisions
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==Crystal structure of decarboxylated osteocalcin at pH 8.5== | |||
<StructureSection load='8i74' size='340' side='right'caption='[[8i74]], [[Resolution|resolution]] 1.36Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8i74]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I74 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.36Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i74 OCA], [https://pdbe.org/8i74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i74 RCSB], [https://www.ebi.ac.uk/pdbsum/8i74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i74 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/OSTCN_BOVIN OSTCN_BOVIN] Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
An acidic environment in bone is essential for bone metabolism and the production of decarboxylated osteocalcin, which functions as a regulatory hormone of glucose metabolism. Here, we describe the high-resolution X-ray crystal structure of decarboxylated osteocalcin under acidic conditions. Decarboxylated osteocalcin at pH 2.0 retains the alpha-helix structure of native osteocalcin with three gamma-carboxyglutamic acid residues at neutral pH. This implies that decarboxylated osteocalcin is stable under an acidic environment in bone. In addition, site-directed mutagenesis revealed that Glu17 and Glu21 are important for the adiponectin-inducing activity of decarboxylated osteocalcin. These findings suggest that the receptor of decarboxylated osteocalcin responds to the negative charge in helix 1 of osteocalcin. | |||
Structural and mutational analyses of decarboxylated osteocalcin provide insight into its adiponectin-inducing activity.,Mizuguchi M, Yokoyama T, Otani T, Kuribara S, Nabeshima Y, Obita T, Hirata M, Kawano K FEBS Lett. 2023 Jun;597(11):1479-1488. doi: 10.1002/1873-3468.14618. Epub 2023 , Apr 4. PMID:36976525<ref>PMID:36976525</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8i74" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Bos taurus]] | |||
[[Category: Large Structures]] | |||
[[Category: Mizuguchi M]] | |||
[[Category: Nabeshima Y]] | |||
[[Category: Obita T]] | |||
[[Category: Yokoyama T]] | |||
Latest revision as of 13:13, 21 August 2024
Crystal structure of decarboxylated osteocalcin at pH 8.5
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