8inh: Difference between revisions
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The | ==ZjOGT3, flavonoid 7,4'-di-O-glycosyltransferase== | ||
<StructureSection load='8inh' size='340' side='right'caption='[[8inh]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8inh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ziziphus_jujuba_var._spinosa Ziziphus jujuba var. spinosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8INH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8INH FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8inh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8inh OCA], [https://pdbe.org/8inh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8inh RCSB], [https://www.ebi.ac.uk/pdbsum/8inh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8inh ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A978UT44_ZIZJJ A0A978UT44_ZIZJJ] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A highly efficient and promiscuous 7,4'-di-O-glycosyltransferase ZjOGT3 was discovered from the medicinal plant Ziziphus jujuba var. spinosa. ZjOGT3 could sequentially catalyse 4'- and 7-O-glycosylation of flavones to produce 7,4'-di-O-glycosides with obvious regio-selectivity. For 7,4'-dihydroxyl flavanones and 3-O-glycosylated 7,4'-dihydroxyl flavones, ZjOGT3 selectively catalyses 7-O-glycosylation. The crystal structure of ZjOGT3 was solved. Structural analysis, DFT calculations, MD simulations, and site-directed mutagenesis reveal that the regio-selectivity is mainly controlled by the enzyme microenvironment for 7,4'-dihydroxyl flavones and 3-O-glycosylated 7,4'-dihydroxyl flavones. For 7,4'-dihydroxyl flavanones, the selectivity is mainly controlled by intrinsic reactivity. ZjOGT3 is the first plant flavonoid 7,4'-di-O-glycosyltransferase with a crystal structure. This work could help understand the catalytic mechanisms of multi-site glycosyltransferases and provides an efficient approach to synthesise O-glycosides with medicinal potential. | |||
Functional characterization, structural basis, and regio-selectivity control of a promiscuous flavonoid 7,4'-di-O-glycosyltransferase from Ziziphus jujuba var. spinosa.,Wang ZL, Wei W, Wang HD, Zhou JJ, Wang HT, Chen K, Wang RS, Li FD, Qiao X, Zhou H, Liang Y, Ye M Chem Sci. 2023 Mar 29;14(16):4418-4425. doi: 10.1039/d2sc06504e. eCollection 2023 , Apr 26. PMID:37123177<ref>PMID:37123177</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8inh" style="background-color:#fffaf0;"></div> | ||
[[Category: Wang | == References == | ||
[[Category: Wang | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Ziziphus jujuba var. spinosa]] | |||
[[Category: Li FD]] | |||
[[Category: Wang HD]] | |||
[[Category: Wang ZL]] | |||
[[Category: Ye M]] | |||
Latest revision as of 07:24, 21 November 2024
ZjOGT3, flavonoid 7,4'-di-O-glycosyltransferase
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