1k9u: Difference between revisions

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[[Image:1k9u.jpg|left|200px]]


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==Crystal Structure of the Calcium-Binding Pollen Allergen Phl p 7 (Polcalcin) at 1.75 Angstroem==
The line below this paragraph, containing "STRUCTURE_1k9u", creates the "Structure Box" on the page.
<StructureSection load='1k9u' size='340' side='right'caption='[[1k9u]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1k9u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phleum_pratense Phleum pratense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K9U FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_1k9u|  PDB=1k9u  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k9u OCA], [https://pdbe.org/1k9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k9u RCSB], [https://www.ebi.ac.uk/pdbsum/1k9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k9u ProSAT]</span></td></tr>
 
</table>
'''Crystal Structure of the Calcium-Binding Pollen Allergen Phl p 7 (Polcalcin) at 1.75 Angstroem'''
== Function ==
 
[https://www.uniprot.org/uniprot/POLC7_PHLPR POLC7_PHLPR]
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
The timothy grass pollen allergen Phl p 7 assembles most of the IgE epitopes of a novel family of 2 EF-hand calcium-binding proteins and therefore represents a diagnostic marker allergen and vaccine candidate for immunotherapy. Here we report the first three-dimensional structure of a representative of the 2 EF-hand allergen family, Phl p 7, in the calcium-bound form. The protein occurs as a novel dimer assembly with unique features: in contrast to well known EF-hand proteins such as calmodulin, parvalbumin or the S100 proteins, Phl p 7 adopts an extended conformation. Two protein monomers assemble in a head-to-tail arrangement with domain-swapped EF-hand pairing. The intertwined dimer adopts a barrel-like structure with an extended hydrophobic cavity providing a ligand-binding site. Calcium binding acts as a conformational switch between an open and a closed dimeric form of Phl p 7. These findings are interesting in the context of lipid- and calcium-dependent pollen tube growth. Furthermore, the structure of Phl p 7 allows for the rational development of vaccine strategies for treatment of sensitized allergic patients.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k9/1k9u_consurf.spt"</scriptWhenChecked>
1K9U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phleum_pratense Phleum pratense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9U OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
The cross-reactive calcium-binding pollen allergen, Phl p 7, reveals a novel dimer assembly., Verdino P, Westritschnig K, Valenta R, Keller W, EMBO J. 2002 Oct 1;21(19):5007-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12356717 12356717]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k9u ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Phleum pratense]]
[[Category: Phleum pratense]]
[[Category: Single protein]]
[[Category: Keller W]]
[[Category: Keller, W.]]
[[Category: Valenta R]]
[[Category: Valenta, R.]]
[[Category: Verdino P]]
[[Category: Verdino, P.]]
[[Category: Westritschnig K]]
[[Category: Westritschnig, K.]]
[[Category: Calcium-binding]]
[[Category: Cross-reactivity]]
[[Category: Ef-hand]]
[[Category: Pollen allergen]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:29:03 2008''

Latest revision as of 13:25, 13 March 2024

Crystal Structure of the Calcium-Binding Pollen Allergen Phl p 7 (Polcalcin) at 1.75 Angstroem

1k9u, resolution 1.75Å

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