8ofv: Difference between revisions
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==Human adenovirus type 53 fiber-knob protein complexed with sialic acid== | |||
<StructureSection load='8ofv' size='340' side='right'caption='[[8ofv]], [[Resolution|resolution]] 1.77Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8ofv]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_53 Human adenovirus 53]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OFV FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=P4G:1-ETHOXY-2-(2-ETHOXYETHOXY)ETHANE'>P4G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene>, <scene name='pdbligand=SLB:5-N-ACETYL-BETA-D-NEURAMINIC+ACID'>SLB</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ofv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ofv OCA], [https://pdbe.org/8ofv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ofv RCSB], [https://www.ebi.ac.uk/pdbsum/8ofv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ofv ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/E5RWD1_9ADEN E5RWD1_9ADEN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Human adenoviruses (HAdV) are widespread pathogens causing usually mild infections. The Species D (HAdV-D) cause gastrointestinal tract infections and epidemic keratoconjunctivitis (EKC). Despite being significant pathogens, knowledge around HAdV-D mechanism of cell infection is lacking. Sialic acid (SA) usage has been proposed as a cell infection mechanism for EKC causing HAdV-D. Here we highlight an important role for SA engagement by many HAdV-D. We provide apo state crystal structures of 7 previously undetermined HAdV-D fiber-knob proteins, and structures of HAdV-D25, D29, D30 and D53 fiber-knob proteins in complex with SA. Biologically, we demonstrate that removal of cell surface SA reduced infectivity of HAdV-C5 vectors pseudotyped with HAdV-D fiber-knob proteins, whilst engagement of the classical HAdV receptor CAR was variable. Our data indicates variable usage of SA and CAR across HAdV-D. Better defining these interactions will enable improved development of antivirals and engineering of the viruses into refined therapeutic vectors. | |||
Broad sialic acid usage amongst species D human adenovirus.,Mundy RM, Baker AT, Bates EA, Cunliffe TG, Teijeira-Crespo A, Moses E, Rizkallah PJ, Parker AL Npj Viruses. 2023;1(1):1. doi: 10.1038/s44298-023-00001-5. Epub 2023 Sep 26. PMID:38665237<ref>PMID:38665237</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8ofv" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Human adenovirus 53]] | |||
[[Category: Large Structures]] | |||
[[Category: Baker AT]] | |||
[[Category: Mundy RM]] | |||
[[Category: Parker AL]] | |||
[[Category: Rizkallah PJ]] | |||
