1w31: Difference between revisions

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New page: left|200px<br /> <applet load="1w31" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w31, resolution 1.9Å" /> '''YEAST 5-AMINOLAEVULI...
 
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[[Image:1w31.gif|left|200px]]<br />
<applet load="1w31" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1w31, resolution 1.9&Aring;" />
'''YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE 5-HYDROXYLAEVULINIC ACID COMPLEX'''<br />


==Overview==
==YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE 5-HYDROXYLAEVULINIC ACID COMPLEX==
The X-ray structure of the enzyme 5-aminolaevulinic acid dehydratase, (ALAD) from yeast complexed with the competitive inhibitor, 5-hydroxylaevulinic acid has been determined at a resolution of 1.9 A. The, structure shows that the inhibitor is bound by a Schiff-base link to one, of the invariant active-site lysine residues (Lys263). The inhibitor, appears to bind in two well defined conformations and the interactions, made by it suggest that it is a very close analogue of the substrate, 5-aminolaevulinic acid (ALA).
<StructureSection load='1w31' size='340' side='right'caption='[[1w31]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1w31]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W31 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SHO:5-HYDROXYLAEVULINIC+ACID'>SHO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w31 OCA], [https://pdbe.org/1w31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w31 RCSB], [https://www.ebi.ac.uk/pdbsum/1w31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w31 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HEM2_YEAST HEM2_YEAST] Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w3/1w31_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w31 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The X-ray structure of the enzyme 5-aminolaevulinic acid dehydratase (ALAD) from yeast complexed with the competitive inhibitor 5-hydroxylaevulinic acid has been determined at a resolution of 1.9 A. The structure shows that the inhibitor is bound by a Schiff-base link to one of the invariant active-site lysine residues (Lys263). The inhibitor appears to bind in two well defined conformations and the interactions made by it suggest that it is a very close analogue of the substrate 5-aminolaevulinic acid (ALA).


==About this Structure==
Structure of yeast 5-aminolaevulinic acid dehydratase complexed with the inhibitor 5-hydroxylaevulinic acid.,Erskine PT, Coates L, Newbold R, Brindley AA, Stauffer F, Beaven GD, Gill R, Coker A, Wood SP, Warren MJ, Shoolingin-Jordan PM, Neier R, Cooper JB Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1222-6. Epub 2005, Aug 16. PMID:16131755<ref>PMID:16131755</ref>
1W31 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with ZN and SHO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W31 OCA]].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of yeast 5-aminolaevulinic acid dehydratase complexed with the inhibitor 5-hydroxylaevulinic acid., Erskine PT, Coates L, Newbold R, Brindley AA, Stauffer F, Beaven GD, Gill R, Coker A, Wood SP, Warren MJ, Shoolingin-Jordan PM, Neier R, Cooper JB, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1222-6. Epub 2005, Aug 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16131755 16131755]
</div>
<div class="pdbe-citations 1w31" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Porphobilinogen synthase|Porphobilinogen synthase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Beaven GDE]]
[[Category: Beaven, G.D.E.]]
[[Category: Brindley AA]]
[[Category: Brindley, A.A.]]
[[Category: Coates L]]
[[Category: Coates, L.]]
[[Category: Cooper JB]]
[[Category: Cooper, J.B.]]
[[Category: Erskine PT]]
[[Category: Erskine, P.T.]]
[[Category: Gill R]]
[[Category: Gill, R.]]
[[Category: Neier R]]
[[Category: Neier, R.]]
[[Category: Newbold R]]
[[Category: Newbold, R.]]
[[Category: Shoolingin-Jordan PM]]
[[Category: Shoolingin-Jordan, P.M.]]
[[Category: Stauffer F]]
[[Category: Stauffer, F.]]
[[Category: Warren MJ]]
[[Category: Warren, M.J.]]
[[Category: Wood SP]]
[[Category: Wood, S.P.]]
[[Category: SHO]]
[[Category: ZN]]
[[Category: aldolase]]
[[Category: dehydratase]]
[[Category: heme biosynthesis]]
[[Category: lyase]]
[[Category: tetrapyrrole synthesis]]
[[Category: tim barrel]]
[[Category: zinc]]
 
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Latest revision as of 07:41, 23 October 2024

YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE 5-HYDROXYLAEVULINIC ACID COMPLEX

1w31, resolution 1.90Å

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