8oo1: Difference between revisions
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==Wide inward-open liganded UraA in complex with a conformation-selective synthetic nanobody== | |||
<StructureSection load='8oo1' size='340' side='right'caption='[[8oo1]], [[Resolution|resolution]] 3.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8oo1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OO1 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.7Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8oo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8oo1 OCA], [https://pdbe.org/8oo1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8oo1 RCSB], [https://www.ebi.ac.uk/pdbsum/8oo1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8oo1 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/URAA_ECOLI URAA_ECOLI] Transport of uracil in the cell.<ref>PMID:7721693</ref> <ref>PMID:21423164</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Uptake of nucleobases and ascorbate is an essential process in all living organisms mediated by SLC23 transport proteins. These transmembrane carriers operate via the elevator alternating-access mechanism, and are composed of two rigid domains whose relative motion drives transport. The lack of large conformational changes within these domains suggests that the interdomain-linkers act as flexible tethers. Here, we show that interdomain-linkers are not mere tethers, but have a key regulatory role in dictating the conformational space of the transporter and defining the rotation axis of the mobile transport domain. By resolving a wide inward-open conformation of the SLC23 elevator transporter UraA and combining biochemical studies using a synthetic nanobody as conformational probe with hydrogen-deuterium exchange mass spectrometry, we demonstrate that interdomain-linkers control the function of transport proteins by influencing substrate affinity and transport rate. These findings open the possibility to allosterically modulate the activity of elevator proteins by targeting their linkers. | |||
Interdomain-linkers control conformational transitions in the SLC23 elevator transporter UraA.,Kuhn BT, Zoller J, Zimmermann I, Gemeinhardt T, Ozkul DH, Langer JD, Seeger MA, Geertsma ER Nat Commun. 2024 Aug 30;15(1):7518. doi: 10.1038/s41467-024-51814-8. PMID:39209842<ref>PMID:39209842</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8oo1" style="background-color:#fffaf0;"></div> | ||
[[Category: Geertsma | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli O157:H7]] | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Geertsma ER]] | |||
[[Category: Kuhn BT]] | |||
Latest revision as of 06:21, 11 September 2024
Wide inward-open liganded UraA in complex with a conformation-selective synthetic nanobody
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