8opr: Difference between revisions
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The | ==Structure of the EA1 surface layer of Bacillus anthracis== | ||
<StructureSection load='8opr' size='340' side='right'caption='[[8opr]], [[Resolution|resolution]] 1.81Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8opr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OPR FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.811Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8opr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8opr OCA], [https://pdbe.org/8opr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8opr RCSB], [https://www.ebi.ac.uk/pdbsum/8opr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8opr ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/SLAP2_BACAN SLAP2_BACAN] The S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of bacteria. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The Gram-positive spore-forming bacterium Bacillus anthracis is the causative agent of anthrax, a deadly disease mostly affecting wildlife and livestock, as well as representing a bioterrorism threat. Its cell surface is covered by the mutually exclusive S-layers Sap and EA1, found in early and late growth phases, respectively. Here we report the nanobody-based structural characterization of EA1 and its native lattice contacts. The EA1 assembly domain consists of 6 immunoglobulin-like domains, where three calcium-binding sites structure interdomain contacts that allow monomers to adopt their assembly-competent conformation. Nanobody-induced depolymerization of EA1 S-layers results in surface defects, membrane blebbing and cell lysis under hypotonic conditions, indicating that S-layers provide additional mechanical stability to the cell wall. Taken together, we report a complete model of the EA1 S-layer and present a set of nanobodies that may have therapeutic potential against Bacillus anthracis. | |||
Structure and function of the EA1 surface layer of Bacillus anthracis.,Sogues A, Fioravanti A, Jonckheere W, Pardon E, Steyaert J, Remaut H Nat Commun. 2023 Nov 3;14(1):7051. doi: 10.1038/s41467-023-42826-x. PMID:37923757<ref>PMID:37923757</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Remaut | <div class="pdbe-citations 8opr" style="background-color:#fffaf0;"></div> | ||
[[Category: Sogues | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bacillus anthracis]] | |||
[[Category: Lama glama]] | |||
[[Category: Large Structures]] | |||
[[Category: Remaut H]] | |||
[[Category: Sogues A]] | |||
Latest revision as of 14:45, 6 November 2024
Structure of the EA1 surface layer of Bacillus anthracis
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