8os6: Difference between revisions
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==Structure of a GFRA1/GDNF LICAM complex== | |||
<StructureSection load='8os6' size='340' side='right'caption='[[8os6]], [[Resolution|resolution]] 2.66Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8os6]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OS6 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.66Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8os6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8os6 OCA], [https://pdbe.org/8os6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8os6 RCSB], [https://www.ebi.ac.uk/pdbsum/8os6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8os6 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q98TT9_DANRE Q98TT9_DANRE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Glial-cell line derived neurotrophic factor (GDNF) bound to its co-receptor GFRalpha1 stimulates the RET receptor tyrosine kinase, promoting neuronal survival and neuroprotection. The GDNF-GFRalpha1 complex also supports synaptic cell adhesion independently of RET. Here, we describe the structure of a decameric GDNF-GFRalpha1 assembly determined by crystallography and electron microscopy, revealing two GFRalpha1 pentamers bridged by five GDNF dimers. We reconsitituted the assembly between adhering liposomes and used cryo-electron tomography to visualize how the complex fulfils its membrane adhesion function. The GFRalpha1:GFRalpha1 pentameric interface was further validated both in vitro by native PAGE and in cellulo by cell-clustering and dendritic spine assays. Finally, we provide biochemical and cell-based evidence that RET and heparan sulfate cooperate to prevent assembly of the adhesion complex by competing for the adhesion interface. Our results provide a mechanistic framework to understand GDNF-driven cell adhesion, its relationship to trophic signalling, and the central role played by GFRalpha1. | |||
Architecture and regulation of a GDNF-GFRalpha1 synaptic adhesion assembly.,Houghton FM, Adams SE, Rios AS, Masino L, Purkiss AG, Briggs DC, Ledda F, McDonald NQ Nat Commun. 2023 Nov 20;14(1):7551. doi: 10.1038/s41467-023-43148-8. PMID:37985758<ref>PMID:37985758</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8os6" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Danio rerio]] | |||
[[Category: Large Structures]] | |||
[[Category: Adams SE]] | |||
[[Category: Briggs DC]] | |||
[[Category: Houghton FM]] | |||
[[Category: McDonald NQ]] | |||
Latest revision as of 07:33, 21 November 2024
Structure of a GFRA1/GDNF LICAM complex
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