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==RNA APTAMER COMPLEXED WITH CITRULLINE, NMR==
The line below this paragraph, containing "STRUCTURE_1kod", creates the "Structure Box" on the page.
<StructureSection load='1kod' size='340' side='right'caption='[[1kod]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1kod]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KOD FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIR:CITRULLINE'>CIR</scene></td></tr>
{{STRUCTURE_1kod| PDB=1kod  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kod OCA], [https://pdbe.org/1kod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kod RCSB], [https://www.ebi.ac.uk/pdbsum/1kod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kod ProSAT]</span></td></tr>
 
</table>
'''RNA APTAMER COMPLEXED WITH CITRULLINE, NMR'''
<div style="background-color:#fffaf0;">
 
== Publication Abstract from PubMed ==
 
==Overview==
In a previous study, an RNA aptamer for the specific recognition of arginine was evolved from a parent sequence that bound citrulline specifically. The two RNAs differ at only 3 positions out of 44. The solution structures of the two aptamers complexed to their cognate amino acids have now been determined by two-dimensional nuclear magnetic resonance spectroscopy. Both aptamers contain two asymmetrical internal loops that are not well ordered in the free RNA but that fold into a compact structure upon ligand binding. Those nucleotides common to both RNAs include a conserved cluster of purine residues, three of which form an uneven plane containing a G:G pair, and two other residues nearly perpendicular to that surface. Two of the three variant nucleotides are stacked on the cluster of purines and form a triple contact to the amino acid side chain, whereas the edge of the third variant nucleotide is capping the binding pocket.
In a previous study, an RNA aptamer for the specific recognition of arginine was evolved from a parent sequence that bound citrulline specifically. The two RNAs differ at only 3 positions out of 44. The solution structures of the two aptamers complexed to their cognate amino acids have now been determined by two-dimensional nuclear magnetic resonance spectroscopy. Both aptamers contain two asymmetrical internal loops that are not well ordered in the free RNA but that fold into a compact structure upon ligand binding. Those nucleotides common to both RNAs include a conserved cluster of purine residues, three of which form an uneven plane containing a G:G pair, and two other residues nearly perpendicular to that surface. Two of the three variant nucleotides are stacked on the cluster of purines and form a triple contact to the amino acid side chain, whereas the edge of the third variant nucleotide is capping the binding pocket.


==About this Structure==
Structural basis of ligand discrimination by two related RNA aptamers resolved by NMR spectroscopy.,Yang Y, Kochoyan M, Burgstaller P, Westhof E, Famulok M Science. 1996 May 31;272(5266):1343-7. PMID:8650546<ref>PMID:8650546</ref>
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOD OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structural basis of ligand discrimination by two related RNA aptamers resolved by NMR spectroscopy., Yang Y, Kochoyan M, Burgstaller P, Westhof E, Famulok M, Science. 1996 May 31;272(5266):1343-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8650546 8650546]
</div>
[[Category: Burgstaller, P.]]
<div class="pdbe-citations 1kod" style="background-color:#fffaf0;"></div>
[[Category: Famulok, M.]]
== References ==
[[Category: Kochoyan, M.]]
<references/>
[[Category: Westhof, E.]]
__TOC__
[[Category: Yang, Y S.]]
</StructureSection>
[[Category: In vitro selected rna]]
[[Category: Large Structures]]
[[Category: Rna aptamer]]
[[Category: Burgstaller P]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:58:52 2008''
[[Category: Famulok M]]
[[Category: Kochoyan M]]
[[Category: Westhof E]]
[[Category: Yang YS]]

Latest revision as of 07:56, 15 November 2023

RNA APTAMER COMPLEXED WITH CITRULLINE, NMR

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