8owx: Difference between revisions

Latest revision as of 07:41, 3 July 2024

Crystal Structure of METTL6 bound to SAH

Structural highlights

8owx is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.601Å
Ligands:	B3P, SAH
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

METL6_HUMAN S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Ser), including tRNA(Ser)(UGA) and tRNA(Ser)(GCU) (PubMed:32923617, PubMed:34268557, PubMed:34862464, PubMed:34922197). Interaction with SARS1/SerRS is required for N(3)-methylcytidine methylation (PubMed:34268557).^[1] ^[2] ^[3] ^[4]

References

↑ Ignatova VV, Kaiser S, Ho JSY, Bing X, Stolz P, Tan YX, Lee CL, Gay FPH, Lastres PR, Gerlini R, Rathkolb B, Aguilar-Pimentel A, Sanz-Moreno A, Klein-Rodewald T, Calzada-Wack J, Ibragimov E, Valenta M, Lukauskas S, Pavesi A, Marschall S, Leuchtenberger S, Fuchs H, Gailus-Durner V, de Angelis MH, Bultmann S, Rando OJ, Guccione E, Kellner SM, Schneider R. METTL6 is a tRNA m(3)C methyltransferase that regulates pluripotency and tumor cell growth. Sci Adv. 2020 Aug 26;6(35):eaaz4551. PMID:32923617 doi:10.1126/sciadv.aaz4551
↑ Mao XL, Li ZH, Huang MH, Wang JT, Zhou JB, Li QR, Xu H, Wang XJ, Zhou XL. Mutually exclusive substrate selection strategy by human m3C RNA transferases METTL2A and METTL6. Nucleic Acids Res. 2021 Aug 20;49(14):8309-8323. PMID:34268557 doi:10.1093/nar/gkab603
↑ Chen R, Zhou J, Liu L, Mao XL, Zhou X, Xie W. Crystal structure of human METTL6, the m(3)C methyltransferase. Commun Biol. 2021 Dec 3;4(1):1361. PMID:34862464 doi:10.1038/s42003-021-02890-9
↑ Li S, Zhou H, Liao S, Wang X, Zhu Z, Zhang J, Xu C. Structural basis for METTL6-mediated m3C RNA methylation. Biochem Biophys Res Commun. 2022 Jan 22;589:159-164. PMID:34922197 doi:10.1016/j.bbrc.2021.12.013

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Ignatova VV, Kaiser S, Ho JSY, Bing X, Stolz P, Tan YX, Lee CL, Gay FPH, Lastres PR, Gerlini R, Rathkolb B, Aguilar-Pimentel A, Sanz-Moreno A, Klein-Rodewald T, Calzada-Wack J, Ibragimov E, Valenta M, Lukauskas S, Pavesi A, Marschall S, Leuchtenberger S, Fuchs H, Gailus-Durner V, de Angelis MH, Bultmann S, Rando OJ, Guccione E, Kellner SM, Schneider R. METTL6 is a tRNA m(3)C methyltransferase that regulates pluripotency and tumor cell growth. Sci Adv. 2020 Aug 26;6(35):eaaz4551. PMID:32923617 doi:10.1126/sciadv.aaz4551

[2] Mao XL, Li ZH, Huang MH, Wang JT, Zhou JB, Li QR, Xu H, Wang XJ, Zhou XL. Mutually exclusive substrate selection strategy by human m3C RNA transferases METTL2A and METTL6. Nucleic Acids Res. 2021 Aug 20;49(14):8309-8323. PMID:34268557 doi:10.1093/nar/gkab603

[3] Chen R, Zhou J, Liu L, Mao XL, Zhou X, Xie W. Crystal structure of human METTL6, the m(3)C methyltransferase. Commun Biol. 2021 Dec 3;4(1):1361. PMID:34862464 doi:10.1038/s42003-021-02890-9

[4] Li S, Zhou H, Liao S, Wang X, Zhu Z, Zhang J, Xu C. Structural basis for METTL6-mediated m3C RNA methylation. Biochem Biophys Res Commun. 2022 Jan 22;589:159-164. PMID:34922197 doi:10.1016/j.bbrc.2021.12.013

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[2]

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[4]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8owx is ON HOLD
+==Crystal Structure of METTL6 bound to SAH==
+<StructureSection load='8owx' size='340' side='right'caption='[[8owx]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8owx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OWX FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.601&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8owx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8owx OCA], [https://pdbe.org/8owx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8owx RCSB], [https://www.ebi.ac.uk/pdbsum/8owx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8owx ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/METL6_HUMAN METL6_HUMAN] S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Ser), including tRNA(Ser)(UGA) and tRNA(Ser)(GCU) (PubMed:32923617, PubMed:34268557, PubMed:34862464, PubMed:34922197). Interaction with SARS1/SerRS is required for N(3)-methylcytidine methylation (PubMed:34268557).<ref>PMID:32923617</ref> <ref>PMID:34268557</ref> <ref>PMID:34862464</ref> <ref>PMID:34922197</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Basu S]]
+[[Category: Dolce LG]]
+[[Category: Kowalinski E]]
+[[Category: Throll P]]

8owx: Difference between revisions

Latest revision as of 07:41, 3 July 2024

Crystal Structure of METTL6 bound to SAH

Structural highlights

Function

References

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