5m2e: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5m2e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M2E FirstGlance]. <br>
<table><tr><td colspan='2'>[[5m2e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M2E FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m2e OCA], [https://pdbe.org/5m2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m2e RCSB], [https://www.ebi.ac.uk/pdbsum/5m2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m2e ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m2e OCA], [https://pdbe.org/5m2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m2e RCSB], [https://www.ebi.ac.uk/pdbsum/5m2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m2e ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/IDH_PSEAB IDH_PSEAB]  
[https://www.uniprot.org/uniprot/IDH_PSEAB IDH_PSEAB]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The glyoxylate shunt bypasses the oxidative decarboxylation steps of the tricarboxylic acid (TCA) cycle, thereby conserving carbon skeletons for gluconeogenesis and biomass production. In Escherichia coli, carbon flux is redirected through the first enzyme of the glyoxylate shunt, isocitrate lyase (ICL), following phosphorylation and inactivation of the TCA cycle enzyme, isocitrate dehydrogenase (ICD), by the kinase/phosphatase, AceK. In contrast, mycobacterial species lack AceK and employ a phosphorylation-insensitive isocitrate dehydrogenase (IDH), which is allosterically activated by the product of ICL activity, glyoxylate. However, Pseudomonas aeruginosa, expresses IDH, ICD, ICL, and AceK, raising the question of how these enzymes are regulated to ensure proper flux distribution between the competing pathways. Here, we present the structure, kinetics, and regulation of ICL, IDH, and ICD from P. aeruginosa We found that flux partitioning is coordinated through reciprocal regulation of these enzymes, linking distribution of carbon flux to availability of the key gluconeogenic precursors, oxaloacetate and pyruvate. Specifically, a greater abundance of these metabolites activated IDH and inhibited ICL, leading to increased TCA cycle flux. Regulation was also exerted through AceK-dependent phosphorylation of ICD; high levels of acetyl-CoA (which would be expected to accumulate when oxaloacetate is limiting) stimulated the kinase activity of AceK, whereas high levels of oxaloacetate stimulated its phosphatase activity. In summary, the TCA cycle-glyoxylate shunt branch-point in P. aeruginosa has a complex enzymology which is profoundly different to that in other species characterized to date. Presumably, this reflects its predilection for consuming fatty acids, especially during infection scenarios.
Gluconeogenic precursor availability regulates flux through the glyoxylate shunt in Pseudomonas aeruginosa.,Crousilles A, Dolan SK, Brear P, Chirgadze DY, Welch M J Biol Chem. 2018 Jul 20. pii: RA118.004514. doi: 10.1074/jbc.RA118.004514. PMID:30030382<ref>PMID:30030382</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5m2e" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Isocitrate dehydrogenase 3D structures|Isocitrate dehydrogenase 3D structures]]
*[[Isocitrate dehydrogenase 3D structures|Isocitrate dehydrogenase 3D structures]]
== References ==
<references/>
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</StructureSection>
</StructureSection>
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