1kwh: Difference between revisions

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[[Image:1kwh.jpg|left|200px]]


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==Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.==
The line below this paragraph, containing "STRUCTURE_1kwh", creates the "Structure Box" on the page.
<StructureSection load='1kwh' size='340' side='right'caption='[[1kwh]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1kwh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_sp._A1 Sphingomonas sp. A1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KWH FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
{{STRUCTURE_1kwh|  PDB=1kwh  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kwh OCA], [https://pdbe.org/1kwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kwh RCSB], [https://www.ebi.ac.uk/pdbsum/1kwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kwh ProSAT]</span></td></tr>
 
</table>
'''Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.'''
== Function ==
 
[https://www.uniprot.org/uniprot/Q9KWT5_SPHSX Q9KWT5_SPHSX]
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
Sphingomonas sp. A1 possesses a high molecular mass (average 25,700 Da) alginate uptake system mediated by a novel pit-dependent ABC transporter. The X-ray crystallographic structure of AlgQ2 (57,200 Da), an alginate-binding protein in the system, was determined by the multiple isomorphous replacement method and refined at 2.0 A resolution with a final R-factor of 18.3% for 15 to 2.0 A resolution data. The refined structure of AlgQ2 was comprised of 492 amino acid residues, 172 water molecules, and one calcium ion. AlgQ2 was composed of two globular domains with a deep cleft between them, which is expected to be the alginate-binding site. The overall structure is basically similar to that of maltose/maltodextrin-binding protein, except for the presence of an N2-subdomain. The entire calcium ion-binding site is similar to the site in the EF-hand motif, but comprises a ten residue loop. This calcium ion-binding site is about 40 A away from the alginate-binding site.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kw/1kwh_consurf.spt"</scriptWhenChecked>
1KWH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sphingomonas_sp. Sphingomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KWH OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1 at 2.0A resolution., Momma K, Mikami B, Mishima Y, Hashimoto W, Murata K, J Mol Biol. 2002 Mar 8;316(5):1051-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11884143 11884143]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kwh ConSurf].
[[Category: Single protein]]
<div style="clear:both"></div>
[[Category: Sphingomonas sp.]]
__TOC__
[[Category: Hashimoto, W.]]
</StructureSection>
[[Category: Mikami, B.]]
[[Category: Large Structures]]
[[Category: Mishima, Y.]]
[[Category: Sphingomonas sp. A1]]
[[Category: Momma, K.]]
[[Category: Hashimoto W]]
[[Category: Murata, K.]]
[[Category: Mikami B]]
[[Category: Alginate]]
[[Category: Mishima Y]]
[[Category: Binding protein]]
[[Category: Momma K]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:15:13 2008''
[[Category: Murata K]]
Retrieved from "https://proteopedia.org/1kwh"