8b6w: Difference between revisions
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New page: ==Mpf2Ba1 pore== <StructureSection load='8b6w' size='340' side='right'caption='8b6w, resolution 2.60Å' scene=''> == Structural highlights == <table><tr><td colspa... |
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[8b6w]] is a 21 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_monteilii Pseudomonas monteilii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B6W FirstGlance]. <br> | <table><tr><td colspan='2'>[[8b6w]] is a 21 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_monteilii Pseudomonas monteilii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B6W FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.6Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b6w OCA], [https://pdbe.org/8b6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b6w RCSB], [https://www.ebi.ac.uk/pdbsum/8b6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b6w ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b6w OCA], [https://pdbe.org/8b6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b6w RCSB], [https://www.ebi.ac.uk/pdbsum/8b6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b6w ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/A0A4Y8SM08_9PSED A0A4Y8SM08_9PSED] | [https://www.uniprot.org/uniprot/A0A4Y8SM08_9PSED A0A4Y8SM08_9PSED] | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The broad adoption of transgenic crops has revolutionized agriculture. However, resistance to insecticidal proteins by agricultural pests poses a continuous challenge to maintaining crop productivity and new proteins are urgently needed to replace those utilized for existing transgenic traits. We identified an insecticidal membrane attack complex/perforin (MACPF) protein, Mpf2Ba1, with strong activity against the devastating coleopteran pest western corn rootworm (WCR) and a novel site of action. Using an integrative structural biology approach, we determined monomeric, pre-pore and pore structures, revealing changes between structural states at high resolution. We discovered an assembly inhibition mechanism, a molecular switch that activates pre-pore oligomerization upon gut fluid incubation and solved the highest resolution MACPF pore structure to-date. Our findings demonstrate not only the utility of Mpf2Ba1 in the development of biotechnology solutions for protecting maize from WCR to promote food security, but also uncover previously unknown mechanistic principles of bacterial MACPF assembly. | |||
Structural journey of an insecticidal protein against western corn rootworm.,Marini G, Poland B, Leininger C, Lukoyanova N, Spielbauer D, Barry JK, Altier D, Lum A, Scolaro E, Ortega CP, Yalpani N, Sandahl G, Mabry T, Klever J, Nowatzki T, Zhao JZ, Sethi A, Kassa A, Crane V, Lu AL, Nelson ME, Eswar N, Topf M, Saibil HR Nat Commun. 2023 Jul 13;14(1):4171. doi: 10.1038/s41467-023-39891-7. PMID:37443175<ref>PMID:37443175</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 8b6w" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
