5b0u: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5b0u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oplophorus_gracilirostris Oplophorus gracilirostris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B0U FirstGlance]. <br>
<table><tr><td colspan='2'>[[5b0u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oplophorus_gracilirostris Oplophorus gracilirostris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B0U FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b0u OCA], [https://pdbe.org/5b0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b0u RCSB], [https://www.ebi.ac.uk/pdbsum/5b0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b0u ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b0u OCA], [https://pdbe.org/5b0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b0u RCSB], [https://www.ebi.ac.uk/pdbsum/5b0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b0u ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/LUCI_OPLGR LUCI_OPLGR] Catalytic subunit of oplophorus-luciferin 2-monooxygenase. Oxidoreductase that converts coelenterazine (the oplophorus luciferin) to coelenteramide under emission of blue light with a maximum at 454 nm. Is also active with bisdeoxycoelenterazine.<ref>PMID:10984608</ref>  
[https://www.uniprot.org/uniprot/LUCI_OPLGR LUCI_OPLGR] Catalytic subunit of oplophorus-luciferin 2-monooxygenase. Oxidoreductase that converts coelenterazine (the oplophorus luciferin) to coelenteramide under emission of blue light with a maximum at 454 nm. Is also active with bisdeoxycoelenterazine.<ref>PMID:10984608</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The 19 kDa protein (KAZ) of Oplophorus luciferase is a catalytic component, that oxidizes coelenterazine (a luciferin) with molecular oxygen to emit light. The crystal structure of the mutated 19 kDa protein (nanoKAZ) was determined at 1.71 A resolution. The structure consists of 11 antiparallel beta-strands forming a beta-barrel that is capped by 4 short alpha-helices. The structure of nanoKAZ is similar to those of fatty acid-binding proteins (FABPs), even though the amino acid sequence similarity was very low between them. The coelenterazine-binding site and the catalytic site for the luminescence reaction might be in a central cavity of the beta-barrel structure.
Crystal structure of nanoKAZ: The mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine.,Tomabechi Y, Hosoya T, Ehara H, Sekine S, Shirouzu M, Inouye S Biochem Biophys Res Commun. 2016 Jan 29;470(1):88-93. doi:, 10.1016/j.bbrc.2015.12.123. Epub 2015 Dec 30. PMID:26746005<ref>PMID:26746005</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5b0u" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==

Latest revision as of 09:06, 20 March 2024

Crystal structure of the mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ)

5b0u, resolution 1.71Å

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