1l5a: Difference between revisions

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[[Image:1l5a.gif|left|200px]]


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==Crystal Structure of VibH, an NRPS Condensation Enzyme==
The line below this paragraph, containing "STRUCTURE_1l5a", creates the "Structure Box" on the page.
<StructureSection load='1l5a' size='340' side='right'caption='[[1l5a]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L5A FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [https://pdbe.org/1l5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [https://www.ebi.ac.uk/pdbsum/1l5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5a ProSAT]</span></td></tr>
{{STRUCTURE_1l5a|  PDB=1l5a  |  SCENE= }}
</table>
 
== Function ==
'''Crystal Structure of VibH, an NRPS Condensation Enzyme'''
[https://www.uniprot.org/uniprot/Q9KTV9_VIBCH Q9KTV9_VIBCH]
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
Nonribosomal peptide synthetases (NRPSs) are large, multidomain enzymes that biosynthesize medically important natural products. We report the crystal structure of the free-standing NRPS condensation (C) domain VibH, which catalyzes amide bond formation in the synthesis of vibriobactin, a Vibrio cholerae siderophore. Despite low sequence identity, NRPS condensation enzymes are structurally related to chloramphenicol acetyltransferase (CAT) and dihydrolipoamide acyltransferases. However, although the latter enzymes are homotrimers, VibH is a monomeric pseudodimer. The VibH structure is representative of both NRPS condensation and epimerization domains, as well as the condensation-variant cyclization domains, which are all expected to be monomers. Surprisingly, despite favorable positioning in the active site, a universally conserved histidine important in CAT and in other C domains is not critical for general base catalysis in VibH.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l5/1l5a_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
1L5A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l5a ConSurf].
The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains., Keating TA, Marshall CG, Walsh CT, Keating AE, Nat Struct Biol. 2002 Jul;9(7):522-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12055621 12055621]
<div style="clear:both"></div>
[[Category: Single protein]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
[[Category: Keating, A E.]]
[[Category: Keating AE]]
[[Category: Keating, T A.]]
[[Category: Keating TA]]
[[Category: Marshall, C G.]]
[[Category: Marshall CG]]
[[Category: Walsh, C T.]]
[[Category: Walsh CT]]
[[Category: Amide synthase]]
[[Category: Nonribosomal peptide synthetase]]
[[Category: Nrps condensation domain]]
[[Category: Vibriobactin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:33:12 2008''

Latest revision as of 07:29, 14 February 2024

Crystal Structure of VibH, an NRPS Condensation Enzyme

1l5a, resolution 2.55Å

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