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New page: left|200px<br /> <applet load="1kso" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kso, resolution 1.70Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:1kso.gif|left|200px]]<br />
<applet load="1kso" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1kso, resolution 1.70&Aring;" />
'''CRYSTAL STRUCTURE OF APO S100A3'''<br />


==Overview==
==CRYSTAL STRUCTURE OF APO S100A3==
S100 proteins are involved in metal-dependent intracellular signalling., Metal-free S100A3, a cysteine-rich Ca(2+)- and Zn(2+)-binding protein, has, been crystallized by vapour diffusion under the strict exclusion of oxygen, and in the absence of divalent metal ions. Metal binding induces large, conformational changes, rendering the apo-S100A3 crystals very sensitive, to various metal compounds. Therefore, the structure was solved by MIRAS, phasing using potassium iodide and xenon derivatives. Iodide replaces a, water molecule at the surface of the S100A3 protein, whereas xenon binds, in a hydrophobic cavity at the dimer interface. Despite significant, non-isomorphism, the combination of both derivatives was sufficient for, structure determination. The overall apo-S100A3 structure resembles the, structures of metal-free S100B and S100A6 solution structures. In contrast, to the NMR structures, the EF-hand loops are well ordered in the, apo-S100A3 crystal structure. In the N-terminal pseudo-EF-hand loop a, water molecule occupies the position of the Ca(2+) ion. The C-terminal, canonical EF-hand loop shows an extended conformation and a different, helix arrangement to other S100/metal complex crystal structures.
<StructureSection load='1kso' size='340' side='right'caption='[[1kso]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1kso]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KSO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KSO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kso OCA], [https://pdbe.org/1kso PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kso RCSB], [https://www.ebi.ac.uk/pdbsum/1kso PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kso ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/S10A3_HUMAN S10A3_HUMAN] Binds both calcium and zinc. May be involved in calcium-dependent cuticle cell differentiation, hair shaft and hair cuticular barrier formation.<ref>PMID:18083705</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/1kso_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kso ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1KSO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KSO OCA].
*[[S100 proteins 3D structures|S100 proteins 3D structures]]
 
== References ==
==Reference==
<references/>
Metal-free MIRAS phasing: structure of apo-S100A3., Mittl PR, Fritz G, Sargent DF, Richmond TJ, Heizmann CW, Grutter MG, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1255-61. Epub 2002, Jul 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12136135 12136135]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Fritz, G.]]
[[Category: Fritz G]]
[[Category: Grutter, M.G.]]
[[Category: Grutter MG]]
[[Category: Heizmann, C.W.]]
[[Category: Heizmann CW]]
[[Category: Mittl, P.R.E]]
[[Category: Mittl PR]]
[[Category: Richmond, T.J.]]
[[Category: Richmond TJ]]
[[Category: Sargent, D.F.]]
[[Category: Sargent DF]]
[[Category: ca2+ binding protein]]
[[Category: ef-hand]]
[[Category: s100]]
[[Category: zn2+ binding protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:54:13 2007''

Latest revision as of 07:27, 14 February 2024

CRYSTAL STRUCTURE OF APO S100A3

1kso, resolution 1.70Å

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